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Bulletin of Botanical Research ›› 2022, Vol. 42 ›› Issue (4): 704-712.doi: 10.7525/j.issn.1673-5102.2022.04.020

• Physiology and Ecology • Previous Articles     Next Articles

Effects of Hormone Combinations, Carbon Source Type and Proliferation Cycle on Embryogenic Callus Proliferation of Pinus koraiensis

Yuhui REN, Shuai NIE, Chunxue PENG, Ling YANG, Hailong SHEN()   

  1. State Key Laboratory of Tree Genetics and Breeding,School of Forestry,Northeast Forestry University,Harbin 150040
  • Received:2021-10-20 Online:2022-07-20 Published:2022-07-15
  • Contact: Hailong SHEN E-mail:shenhl-cf@nefu.edu.cn
  • About author:REN Yunhui(1996—),female,postgraduate,mainly engaged in research of tree tissue culture and plant propagation biology and technology.
  • Supported by:
    Study on Key Technologies of Directional Cultivation of high-quality large-diameter timber of Pinus koraiensis(2017YFD0600601)


In order to solve the poor growth and low somatic embryo production in the embryogenic callus proliferation of Pinus koraiensisP. koraiensis embryogenic callus 1-100 cell line was used as material to explore its effect on the somatic embryogenesis and the changes of physiological and biochemical characteristics by adjusting hormone, carbon source and proliferation culture cycle in proliferation medium. The results were as follows: ①the optimum proliferation condition was 1 mg·L-1 NAA+0.25 mg·L-1 6-BA, the best carbon source was sucrose, and the best culture cycle was 14 days. Under the conditions, the largest number of somatic embryos were obtained(166·g-1); ②in the process of optimizing proliferation culture, the stored substances provided energy for somatic embryogenesis; There was no significant difference in soluble protein mass fraction between hormone types and their concentrations, carbon source types and proliferation culture cycles; ③high CAT activity in callus was harmful to somatic embryogenesis, which might be due to excessive accumulation of toxic metabolites such as H2O2.

Key words: Pinus koraiensis, embryogenic callus, somatic embryogenesis, proliferation medium

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