Abstract: We studied the effects of pre-treatments on seed germination, callus inducement and the plant regeneration of Lolium perenne. The results showed that pre-treatments including seeds drying 35℃ for 3 h, immersion for 16 hours then drying for 48 h and irradiation under 1 000 Gs magnetic field promoted the germination ratios significantly. MS medium was the optimal one in the three media tested. The 6 mg·L^-1 2,4-D was suitable for inducing callus formation from seeds. The medium for callus differentiation into plantlets contained 2 mg·L^-1 cytokinin (6-BA or KT). The basic MS medium adding 0.5 mg·L^-1 NAA was best for regenerated plantlets rooting.

Key words: Lolium perenne, seeds, pre-treatments, callus, plant regeneration