Genetic Diversity and Genetic Structure of the Rare and Endangered Plant Ferula fukanensis

doi:10.7525/j.issn.1673-5102.2023.01.006

Abstract

Abstract:

To clarify the genetic diversity and genetic structure of a rare and the endangered small population of Ferula fukanensis， it is the basis and prerequisite to develop effective conservation and management strategies. The genetic diversity and structure of 87 endangered plants F. fukanensis from three populations based on 10 pairs of SSR primers with good polymorphism and stable amplification were analyzed respectively. The results showed that small population of F. fukanensis had relatively high genetic diversity， the Nei’s gene diversity index（h_S） among the populations was 0.514， the total Nei’s gene diversity index（h_T） was 0.516， the observed heterozygosity（H_o） was 0.881， the expected heterozygosity（H_e） was 0.512， the Shannon information index（I） was 0.836 and the percentage of polymorphic bands（P_PB） was 100%， and the genetic differentiation degree of F. fukanensis was very low（F_st=0.007）， 95.9 % of the variation of F. fukanensis occurred within populations， and there was no significant correlation between genetic distance and geographical distance， 66.7% of the populations suffered from genetic bottlenecks. The results showed that F. fukanensis had abundant genetic variation and high evolutionary potential. Based on the current situation of this wild population， it is suggested to establish a protected area， and carry out in situ conservation and strengthen ex situ conservation measures such as introduction and artificial breeding to assist the conservation of F. fukanensis. This study can provide theoretical support for the conservation of plant resources of F. fukanensis and has important theoretical and practical significance.

Key words: Ferula fukanensis, endangered species, small population, genetic diversity, conservation

CLC Number:

Q949.763.3

Lei YANG, Qiumei CAO, Ying FENG, Wenjun LI. Genetic Diversity and Genetic Structure of the Rare and Endangered Plant Ferula fukanensis[J]. Bulletin of Botanical Research, 2023, 43(1): 51-58.

Figures/Tables 6

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References 56

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位点 Locus	等位基因数 N_a	有效等位基因数 N_e	观测杂合度 H_o	期望杂合度 H_e	多态信息含量 P_IC	香农信息指数 I	近交系数 F	基因分化系数 F_st	基因流 N_m	偏离哈温平衡 HWE
FS1	3.0	2.019	1.000	0.505	0.384	0.715	-0.982	0.000	1 390.875	**
FS2	5.0	1.115	0.104	0.099	0.109	0.237	-0.040	0.018	13.283
FS6	6.0	2.506	0.927	0.594	0.538	1.056	-0.570	0.011	22.872	**
FS9	2.0	1.999	0.990	0.500	0.375	0.693	-0.981	0.000	1 377.750	**
FS12	5.0	2.633	1.000	0.612	0.540	1.100	-0.645	0.010	25.496	**
FS18	5.0	2.902	0.834	0.654	0.630	1.246	-0.275	0.027	8.881	*
FS21	4.0	2.039	1.000	0.509	0.392	0.736	-0.965	0.000	701.813	**
FS36	5.0	2.837	0.979	0.646	0.602	1.191	-0.519	0.007	36.433	**
FS41	2.0	1.999	0.989	0.500	0.375	0.693	-0.978	0.000	1012.125	**
FS45	2.0	1.999	0.985	0.500	0.375	0.693	-0.971	0.000	544.125	**
Mean	3.9	2.205	0.881	0.512	0.432	0.836	-0.693	0.007	513.365

种群 Pop	等位基因数 N_a	有效等位基因数 N_e	香农指数 I	观测杂合度 H_o	期望杂合度 H_e	近交系数 F	多态位点百分率 P_PB /%
Mean	3.233	2.205	0.836	0.881	0.512	-0.693	100
XQ	3.100	2.250	0.841	0.877	0.514	-0.677	100
CYL	2.900	2.118	0.782	0.877	0.495	-0.723	100
XHG	3.700	2.247	0.885	0.889	0.526	-0.678	100

居群 Locality	威氏符号秩次检验（一尾） Wilcoxon signed rank test（one-tailed P-value）			模型检验 Mode-shift test
居群 Locality	无限等位基因模型 IAM	双向突变模型 TPM	逐步突变模型 SMM	模型检验 Mode-shift test
XQ	0.005**	0.042*	0.065	偏态分布 shifted distribution
CYL	0.003**	0.012*	0.065	偏态分布 shifted distribution
XHG	0.009**	0.042*	0.461	正常L-型分布 normal L-shaped distribution

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