Effects of Light Signal on Anthocyanin Biosynthesis and Gene Expression of AmRosea1 Overexpressed 84K Poplar

doi:10.7525/j.issn.1673-5102.2023.06.004

Abstract

Abstract:

To explore the reasons of color change in AmRosea1 overexpressed 84K poplar（Populus alba× P. glandulosa ‘84K’） caused by light signal， the wild type and AmRosea1 overexpressed 84K poplar were used as the materials. The anthocyanin content， chlorophyll content， soluble sugar content and POD activity under LED light， natural light， LED red light， LED blue light， and LED red and blue light treatment were measured， and the changes of physiological indexes of wild type and transgenic lines under different optical signal were determined respectively. The results showed that under different light intensities， compared with the LED light treatment， the leaves of the transgenic lines turned red， and the anthocyanin content and soluble sugar content increased， and the chlorophyll content and POD activity decreased under natural light. After 30 days of different light treatments， the LED red-blue light treatment that the antho-cyanin content increased， the soluble sugar content and POD activity decreased， and the chlorophyll content decreased slightly under LED red and blue light induction compared with LED red and blue light. Based on the experimental results， it was speculated that strong light and red and blue light quality might activate the anthocyanin biosynthesis pathway and accumulate a large amount of anthocyanins in AmRosea1 overexpressed 84K poplar， which in turn the leaves turned red.

Key words: Light intensity, light quality, AmRosea1, 84K poplar, physiological characteristics, gene expression

CLC Number:

S792.11

Huiling YAN, Xinxin ZHANG, Xiyang ZHAO, Guanzheng QU, Zhaoning WANG, Rui HAN. Effects of Light Signal on Anthocyanin Biosynthesis and Gene Expression of AmRosea1 Overexpressed 84K Poplar[J]. Bulletin of Botanical Research, 2023, 43(6): 815-825.

Figures/Tables 9

Table 1

Fig.1

Fig.2

Fig.3

Fig.4

Fig.5

Fig.6

Fig.7

Fig.8

References 25

1	刘恺媛，王茂良，辛海波，等.植物花青素合成与调控研究进展［J］.中国农学通报，2021，37（14）：41-51.
	LIU K Y， WANG M L， XIN H B，et al.Anthocyanin biosynthesis and regulate mechanisms in plants：a review［J］.Chinese Agricultural Science Bulletin，2021，37（14）：41-51.
2	LIU Y， TIKUNOV Y， SCHOUTEN R E，et al.Anthocyanin biosynthesis and degradation mechanisms in Solanaceous vegetables：a review［J］.Frontiers in Chemistry，2018，6：52.
3	YAN H L， PEI X N， ZHANG H，et al.MYB-mediated regulation of anthocyanin biosynthesis［J］.International Journal of Molecular Sciences，2021，22（6）：3103.
4	MAIER A， HOECKER U.COP1/SPA ubiquitin ligase complexes repress anthocyanin accumulation under low light and high light conditions［J］.Plant Signaling & Behavior，2015，10（1）：e970440.
5	马超，冯雅岚，吴姗薇，等.鼓粒期遮光对黑绿豆种皮花青素积累及相关基因表达特性的影响［J］.作物学报，2022，48（11）：2826-2839.
	MA C， FENG Y L， WU S W，et al.Effects of shading at grain filling stages on anthocyanin accumulation and related gene expression characteristics in seed coat of black mung bean［J］.Acta Agronomica Sinica，2022，48（11）：2826-2839.
6	ZHANG Y T， JIANG L Y， LI Y L，et al.Effect of red and blue light on anthocyanin accumulation and differential gene expression in strawberry （Fragaria × ananassa）［J］.Molecules，2018，23（4）：820.
7	王佳淇，何莹钰，韦晓桐，等.LED补光组合对大棚越橘生长发育的影响［J］.园艺学报，2020，47（6）：1183-1193.
	WANG J Q， HE Y Y， WEI X T，et al.Effects of LED supplemental light on the growth and development of blueberry in Greenhouse［J］.Acta Horticulturae Sinica，2020，47（6）：1183-1193.
8	FU Z Z， SHANG H Q， JIANG H，et al.Systematic identification of the light-quality responding anthocyanin synthesis-related transcripts in petunia petals［J］.Horticultural Plant Journal，2020，6（6）：428-438.
9	朱俊杰，王天顺，牙禹，等.莴笋叶片低温下积累花青素对光合作用的影响［J］.南方农业学报，2021，52（1）：180-188.
	ZHU J J， WANG T S，YA Y，et al.Effects of anthocyanin accumulation on photosynthesis in leaves of asparagus lettuce under low temperature［J］.Journal of Southern Agriculture，2021，52（1）：180-188.
10	SHARMA S， HOLME I B， DIONISIO G，et al.Cyanidin based anthocyanin biosynthesis in orange carrot is restored by expression of AmRosea1 and AmDelila，MYB and bHLH transcription factors［J］.Plant Molecular Biology，2020，103（4/5）：443-456.
11	YAN H L， ZHANG X X， LI X，et al.Integrated transcriptome and metabolome analyses reveal the anthocyanin biosynthesis pathway in AmRosea1 overexpression 84K poplar［J］.Frontiers in Bioengineering and Biotechnology，2022，10：911701.
12	WANG H H， WANG X Q， SONG W M，et al. PdMYB118，isolated from a red leaf mutant of Populus deltoids，is a new transcription factor regulating anthocyanin biosynthesis in poplar［J］.Plant Cell Reports，2019，38（8）：927-936.
13	国庆.小黑杨转录因子PsnHDZ01基因调控抗旱耐盐的分子机制研究［D］.哈尔滨：东北林业大学，2022：65-66.
	GUO Q.Molecular mechanism of transcription factor PsnHDZ01 in regulation of drought and salt tolerance in Populus simonii × Populus nigra ［D］.Harbin：Northeast Forestry University，2022：65-66.
14	由继红，董春光，史晓昆.小麦叶片可溶性糖含量测定方法的研究［J］.实验室科学，2021，24（2）：27-29，33.
	YOU J H， DONG C G， SHI X K.Study on determination method of soluble sugar content in wheat leaves［J］.Laboratory Science，2021，24（2）：27-29，33.
15	王伟玲，王展，王晶英.植物过氧化物酶活性测定方法优化［J］.实验室研究与探索，2010，29（4）：21-23.
	WANG W L， WANG Z， WANG J Y.Optimization of determination method of peroxidase activity in plant［J］.Research and Exploration in Laboratory，2010，29（4）：21-23.
16	花扬，吕昕羽，张瑜，等.桃MYB转录因子调控花青素合成的研究进展［J］.现代农业科技，2022，（21）：75-79.
	HUA Y， LÜ X Y， ZHANG Y，et al.Research progress on anthocyanin synthesis regulated by MYB transcription factor in peach modern［J］.Agricultural Science and Technology，2022（21）：75-79.
17	洪艳，武宇薇，宋想，等.光照调控园艺作物花青素苷生物合成的分子机制［J］.园艺学报，2021，48（10）：1983-2000.
	HONG Y， WU Y W， SONG X，et al.Molecular mechanism of light-induced anthocyanin biosynthesis in horticultural crops［J］.Acta Horticulturae Sinica，2021，48（10）：1983-2000.
18	WANG S， LI L X， FANG Y，et al.MdERF1B-MdMYC2 module integrates ethylene and jasmonic acid to regulate the biosynthesis of anthocyanin in apple［J］.Horticulture Research，2022，9：uhac142.
19	宋明，孙梓健，汤青林，等.环境胁迫下大头芥花青素积累及其相关结构基因的表达［J］.中国蔬菜，2012（6）：27-34.
	SONG M， SUN Z J， TANG Q L，et al.Studies on anthocyanin accumulation and related gene expression in big-head mustard〔Brassica juncea（L.）Czern.et Coss.var.megarrhiza Tsen et Lee〕induced by environmental stress［J］.China Vegetables，2012（6）：27-34.
20	郭瀚成，金晓玲.外源蔗糖对3种色系榉树秋季叶色变化的影响［J］.植物生理学报，2020，56（11）：2487-2494.
	GUO H C， JIN X L.Effect of exogenous sucrose on autumn leaf color changes of three color lines of beech trees［J］.Plant Physiology Journal，2020，56（11）：2487-2494.
21	NAGIRA Y， OZEKI Y.A system in which anthocyanin synthesis is induced in regenerated torenia shoots［J］.Journal of Plant Research，2004，117（5）：377-383.
22	吴珍.光质对桔梗叶花青素含量及酶活性的影响［J］.商洛学院学报，2022，36（6）：26-31，79.
	WU Z.Effects of light quality on anthocyanin content and enzyme activity in leaves of Platycodon grandiflorum ［J］.Journal of Shangluo University，2022，36（6）：26-31，79.
23	钟秀来，罗庆，张德军，等.不同光质条件下紫色芹菜花青素响应机理分析［J］.分子植物育种，2021，19（19）：6529-6535.
	ZHONG X L， LUO Q， ZHANG D J，et al.Response mechanism analysis of anthocyanin in purple celery under light quality condition［J］.Molecular Plant Breeding，2021，19（19）：6529-6535.
24	樊颖伦，孙艳茹，卞春松，等.不同光质对紫色马铃薯薯块花青素含量的影响［J］.聊城大学学报（自然科学版），2013，26（4）：76-79.
	FAN Y L， SUN Y R， BIAN C S，et al.Effect of different light quality on anthocyanin content of purple potato cubes［J］.Journal of Liaocheng University （Natural Science Edition），2013，26（4）：76-79.
25	刘雪梅，金晓玲，汪晓丽，等.3类色系榉树叶色表达期色素含量变化规律研究［J］.河南农业大学学报，2014，48（5）：596-601.
	LIU X M， JIN X L， WANG X L，et al.Study on the pigment content change rule in the leaf color expression period of 3 groups of leaf color Zelkova schneideriana ［J］.Journal of Henan Agricultural University，2014，48（5）：596-601.

基因名 Gene	上游引物（5′→3′） Upstream primer（5′→3′）	下游引物（5’→3’） Downstream primer（5’→3’）
AmRosea1	AGAGTATGGTGAAGGGAAATGG	CCGACCTCTTTTGATATTTGGC
IF7MAT	CAATCACTTTCCCATGCACTC	TCACCATTACACTCGGCAAG
FG2	GTCACTCAGGGTTTAGCTCG	CCTATTTATCTCCACCCCAGC
DFR	GGAAACATGCAGAGAAAAGGG	GGAATAAGAGGACCGTGGAAC
ANS	CCAGTAGAGGTGAAGCAAGAG	GACTTGCTCCTTTCTCCACTC
BZ1.1	GACAGGGAGAAAGGTGAGTTG	CATGAGCAGAAAGTGAGGTAGG
Actin	AATACCCCATTGAGCACGG	ACTCACACCATCACCAGAAT

[1]	Chaoyang MA, Tianfeng LIU, Xiaoxue LI, Cancan HUO, Lingling FU, Zailiu LI. Interannual Changes in the Effects of Soil Gravel Content on the Growth and Physiology of Handeliodendron bodinieri Seedlings [J]. Bulletin of Botanical Research, 2023, 43(6): 890-899.
[2]	Shuyao ZHUANG, Hengbo XU, Xiaoyu HU, Shang DAI, Yanni ZHANG. Effects of Saline-alkali Stress on Growth and Physiological Characteristics of Color Leaf Heuchera micrantha ‘Silver Fan’ Seedlings [J]. Bulletin of Botanical Research, 2023, 43(4): 520-530.
[3]	Lei XU, Xiao XU, Qinsong LIU. Effects of Exogenous Salicylic Acid on Antioxidant System and Gene Expression of Davidia involucrata Seedlings under Salt Stress [J]. Bulletin of Botanical Research, 2023, 43(4): 572-581.
[4]	Hong YANG, Lifeng WANG, Longjun DAI, Bingbing GUO. Effects of Tapping Panel Dryness on Mitochondrial Ultrastructure and ROS Metabolism in Barks of Rubber Tree (Hevea brasiliensis) [J]. Bulletin of Botanical Research, 2023, 43(1): 69-75.
[5]	Mengshuo LI, Yingze LIU, Huan LU, Sheng QIANG. Photosynthetic Capacity Differentiation and Gene Transcription in Different Geographical Populations of Arabidopsis thaliana under Common Garden conditions [J]. Bulletin of Botanical Research, 2023, 43(1): 90-99.
[6]	Zhihui HUANG, Yining ZHANG, Nana LI, Baojiang ZHENG, Yuhong ZHANG. Responses of Supplemental UV-B Radiation to Physiological Properties and Secondary Metabolites of Thlaspi arvense [J]. Bulletin of Botanical Research, 2022, 42(6): 1079-1087.
[7]	Bi QIN, Mingyang LIU, Meng WANG, Lifeng WANG, Fei HUANG. Cloning and Expression Analysis of DELLA gene HbRGL1 from Hevea brasiliensis Müll. Arg [J]. Bulletin of Botanical Research, 2022, 42(6): 997-1004.
[8]	Longjun DAI, Mingyang LIU, Jianghua YANG, Kai ZHOU, Bingbing GUO, Hong YANG, Lifeng WANG. Structural and Gene Expression Analysis of a DUF1262 Domain Protein in Latex from Hevea brasiliensis [J]. Bulletin of Botanical Research, 2022, 42(5): 802-810.
[9]	Sisi CHEN, Muhong XIE, Maokai CUI, Wenkai LI, Zhenggang XU, Caixia JIA, Guiyan YANG. Identification of Broussonetia papyrifera Transcription Factor BpbZIP1 and Analysis of Its Response to Cadmium Stress [J]. Bulletin of Botanical Research, 2022, 42(3): 394-402.
[10]	Shanshan ZHANG, Chunming YUAN. Effects of Soil Moisture and Light Intensity on Growth and Photosynthetic Characteristics of Pterospermum kingtungense Seedlings [J]. Bulletin of Botanical Research, 2022, 42(3): 502-511.
[11]	Kaiheng Ma, Jianing He, Muhong Xie, Bin Ren, Qianxue Huang, Guiyan Yang. Cloning and Disease Resistance Response Potential of Walnut JrNPR1 Gene [J]. Bulletin of Botanical Research, 2022, 42(1): 39-46.
[12]	Hua-Feng CHEN, Song-Le FAN, Li-Feng WANG. Structural and Functional Analysis of Gibberellin Signaling Transduction Pathway Gene HbRGA1 in Rubber Tree [J]. Bulletin of Botanical Research, 2021, 41(4): 531-539.
[13]	Sen SHI, Na MIAO, Yu-Tong SHI, Hui-Mei WANG. Effects of Sucrose on Growth and Physiological Characteristics in vitro Plantlets of 84K Poplar [J]. Bulletin of Botanical Research, 2021, 41(1): 74-78.
[14]	LIU Yue, ZHAO Kai, Lü Guan-Bin, JIANG Ting-Bo, ZHOU Bo-Ru. Transcription Factor Gene ERF11 Response to Osmotic Stress in Populus simonii×P.nigra Poplar [J]. Bulletin of Botanical Research, 2020, 40(3): 433-440.
[15]	XIAO Zhi-Peng, YIN Chong-Min, GUO Lian-Jin, WU Yuan-Rong, HU Jin-Ping, LIU Yan-Yan, ZHONG You-Chun, XUE Ping-Ping. Effect of Light Quality on Seed Germination and Seeding Growth of Emmenopterys henryi [J]. Bulletin of Botanical Research, 2020, 40(2): 189-195.