Table 1

Primer sequences involved in this study

Fig.1

Identification of sco2 and parc6 mutants and expression levelsA.Model of gene structures and DNA mutation sites of sco2. Exons（green），introns （black line），and untranslated regions（white） were indicated；B. henotypes of wild type and sco2 mutant after 8 d；C. Genotyping and PCR analysis of genomic DNA isolated from WT and sco2 mutants，primer positions were indicated in figure A；D.Expression of SCO2 gene in WT and sco2 mutants；E.Model of gene structures and positions of T-DNA in the parc6 mutants.Exons（green），introns（black line），and untranslated regions（white） were indicated；F.Phenotypes of wild type and parc6 mutant after 24 d；G.Genotyping and PCR analysis of genomic DNA isolated from WT and parc6 mutants，primer positions were indicated in figure D；H.Expression of PARC6 gene in WT and parc6 mutants

Fig.2

Visualization of chloroplast morphology of mesophyll cells of leaf and cotyledon in different lines by confocal laser microscopyA.WT cotyledon；B.WT true leaf；C. sco2 true leaf；D. parc6，cotyledon；E. parc6，true leaf；F. sl2，true leaf

Fig.3

Abnormal chloroplast division hindered growth of cotyledon and leafA.Cotyledon phenotypes of different lines grew after 5 d；B.Leaf phenotype of different lines grew after 7 d and cotyledon removed

Fig.4

Fresh weight，chlorophyll content and photosyn-thetic fluorescence parameters of cotyledon and true leafA-B.The fresh weight and chlorophyll content；C-D.Chlorophyll fluorescence parameters；*P<0.05，**P<0.0；The same as below

Fig.5

Effect of different concentrations of sucrose treatment on different linesA.Different lines grew on ?MS medium with different concentrations sucrose after 6 d under normal-light conditions（80 μmol·m2·s-1）；B.Chlorophyll fluorescence parameters of different lines grew on different concentrations of sucrose

Fig.6

Fresh weight and chlorophyll content of different lines treated with different concentration of sucroseA.All mutants differed significantly from the wild type；B.Chlorophyll content of different lines grew on different concentrations of sucrose

Fig.7

Analysis of chloroplast thylakoid protein complexA.The thylakoids proteins extracted from 7 d cotyledon of wild type and parc6 mutant and 14 d leaf wild type and parc6 mutant for blue native gel electrophoresis（PSⅡ-SC.PSⅡ super complex；PSⅠ-M.PSⅠ monomer；PSⅡ-D.PSⅡ dimer；LHCⅡ.PSⅡ light-harvesting complex；LHCⅡ-T.PSⅡ light-harvesting complex trimer；LHCⅡ monomer，PSⅡ light-harvesting complex monomer）；B.12.5% 2D SDS-PAGE，the red dashed box indicated the photosystem Ⅱ super complex protein

Fig.8

Phylogenetic tree of the rootless development of PARC6 and SCO2 proteins