Leaf Epidermal Micromorphological Features and Their Systematic Significance of Six Wild Speciesof Camellia chrysantha

doi:10.7525/j.issn.1673-5102.2021.06.001

Abstract

Abstract:

To compare the differences in leaf epidermis micro-morphology of Camellia chrysantha， the light microscope and scanning electron microscope were used to investigate the mature leaf epidermis micro-morphological features of six wild C. chrysantha species in Guangxi Nonggang National Nature Reserve. The results showed that： ①The leaf epidermis was composed of epidermal fine stomata apparatus in six C. chrysantha species， and the stomata were randomly distributed on the leaf abaxial surface. Each stomata had three guard cells with different sizes， which categorized as annular type stomata. There were significant differences in stomatal length， stomatal width， stomatal density， stomatal index and cell density of upper and lower epidermis cell density among six species of C. chrysantha（P<0.05）. ②Cluster analysis results with epidermal traits showed that C. ptilosperma and C. longgangensis were classified into one category， and the other four species were classified into each own category separately. Similar results were shown from the micro-morphological features， such as location of the flat peripheral wall and cell wall of epidermal cells， the ornamentation of cuticle and waxy layer covering epidermal cells， the location of stomata and epidermal cells， the morphology of the surface and inner edge of stomatal outer arches， and the cuticle membrane on the outer edge of stomatal outer arches. ③Filamentous or chain-like wax decoration lines were commonly found on epidermal cells of the six species leaves， which might be used as an important evidence for the classification of cultivated species and wild species of C. chrysantha. ④Wild C. chrysantha species showed strong adaptability to the arid karst habitat based on the leaf epidermal traits， including the distribution of stomata， the existence of cuticle and wax layer， thick epidermal cell wall and sunken stomata.

Key words: Camellia chrysantha, leaf epidermis, micro-morphological features, classification, adaptability

CLC Number:

Q944.56

Li-Qiong ZHU, Dong-Mei QIN, Li-Jun ZHAO, Bin-Sheng DENG, Sheng-Yuan LIU, Chang-Jie JIANG. Leaf Epidermal Micromorphological Features and Their Systematic Significance of Six Wild Speciesof Camellia chrysantha[J]. Bulletin of Botanical Research, 2021, 41(6): 841-850.

Figures/Tables 8

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References 30

1	韦霄，蒋运生，韦记青，等.珍稀濒危植物金花茶地理分布与生境调查研究［J］.生态环境，2007，16（3）：895-899.
	Wei X，Jiang Y S，Wei J Q，et al.Investigation on the geographical distribution and habitat of Camellia nitidissima［J］.Ecology and Environment，2007，16（3）：895-899.
2	张宏达.华夏植物区系的金花茶组［J］.中山大学学报，1979，（3）：69-74.
	Chang H T.Chrysantha，a section of golden camellias from Cathaysian Flora［J］.Acta Scientiarum Naturalium Universitatis Sunyatseni，1979，（3）：69-74.
3	苏宗明，莫新礼.我国金花茶组植物的地理分布［J］.广西植物，1988，8（1）：75-81.
	Su Z M，Mo X L.Geographic distribution of camellia section Chrysantha from China［J］.Guihaia，1988，8（1）：75-81.
4	梁盛业.世界金花茶植物名录［J］.广西林业科学，2007，36（4）：221-223.
	Liang S Y.The world list of camellia［J］.Guangxi Forestry Science，2007，36（4）：221-223.
5	叶创兴，许兆然.关于金花茶组的研究［J］.中山大学学报：自然科学版，1992，31（4）：68-77.
	Ye C X，Xu Z R.A taxonomy of Camellia Sect.Chrysantha Chang［J］.Acta Scifntiarum Naturalium Universitatis Sunyaatseni，1992，31（4）：68-77.
6	闵天禄，张文驹.山茶属古茶组和金花茶组的分类学问题［J］.云南植物研究，1993，15（1）：1-15.
	Ming T L，Zhang W J.On taxonomic problems of Sect.Archecamellia Sealy and Sect.Chrysantha Chang in the genus Camellia［J］.Acta Botanica Yunnanica，1993，15（1）：1-15.
7	唐绍清，施苏华，钟杨，等.基于ITS序列探讨山茶属金花茶组的系统发育关系［J］.广西植物，2004，24（6）：488-492，487.
	Tang S Q，Shi S H，Zhong Y，et al.Phylogenetic relationships of golden camellia（sect.Chrysantha，Camellia） in China：evidence from ITS sequences of nuclear ribosomal DNA［J］.Guihaia，2004，24（6）：488-492，487.
8	施苏华，唐绍清，陈月琴，等.11种金花茶植物的RAPD分析及其系统学意义［J］.植物分类学报，1998，36（4）：317-322.
	Shi S H，Tang S Q，Chen Y Q，et al.Phylogenetic relationships among eleven yellow flowered Camellia species based on random amplified polymorphic DNA［J］.Acta Phytotaxonomica Sinica，1998，36（4）：317-322.
9	肖政，李纪元，李志辉，等.金花茶组物种遗传关系的ISSR分析［J］.林业科学研究，2014，27（1）：71-76.
	Xiao Z，Li J Y，Li Z H，et al.Genetic relationships among species from Camellia Sect.Chrysantha Chang revealed by ISSR analysis［J］.Forest Research，2014，27（1）：71-76.
10	唐文秀，盘波，毛世忠，等.凹脉金花茶和东兴金花茶的繁殖试验研究［J］.西北林学院学报，2009，24（2）：63-67.
	Tang W X，Pan B，Mao S Z，et al.Studies on the reproductive methods of Camellia impressinervis and C.tunghinensis［J］.Journal of Northwest Forestry University，2009，24（2）：63-67.
11	邓荫伟，吴婵榕，郭俊红，等.金花茶扦插育苗试验［J］.分子植物育种，2017，15（7）：2886-2890.
	Deng Y W，Wu C R，Guo J H，et al.Experiment of cutting seedlings of Camellia nitidissima［J］.Molecular Plant Breeding，2017，15（7）：2886-2890.
12	姜丽娜，李纪元，范正琪，等.金花茶组植物花朵内多酚组分含量分析［J］.林业科学研究，2020，33（4）：117-126.
	Jiang L N，Li J Y，Fan Z Q，et al.Content analysis of polyphenols in flowers of yellow Camellia［J］.Forest Research，2020，33（4）：117-126.
13	邹琦丽.广西金花茶叶解剖［J］.广西植物，1987，7（1）：45-47.
	Zou Q L.Observation on leaf structure of camellia Sect. Chrysantha from Guangxi［J］.Guihaia，1987，7（1）：45-47.
14	王任翔，胡长华，梁盛业，等.金花茶组植物叶表皮特征的扫描电镜观察［J］.广西林业科学，2002，31（3）：133-136.
	Wang R X，Hu C H，Liang S Y，et al.Studies on the characters of foliar surface under SEM in Sect. Chrysantha［J］.Guangxi Forestry Science，2002，31（3）：133-136.
15	王任翔，梁盛业，李洁荣，等.金花茶组植物叶表皮特征的扫描电镜观察（Ⅱ）［J］.广西林业科学，2003，32（1）：12-16，6.
	Wang R X，Liang S Y，Li J R，et al.Studies on the characters of foliar surface under SEM in Sect. Chrysantha （Ⅱ）［J］.Guangxi Forestry Science，2003，32（1）：12-16，6.
16	李凤英，唐绍清，梁士楚.山茶属金花茶组金花茶系植物叶表皮形态学研究［J］.广西植物，2013，33（3）：376-382.
	Li F Y，Tang S Q，Liang S C.Leaf epidermal morphology of Ser.Chrysantha（Camellia，Sect.Chrysantha）［J］.Guihaia，2013，33（3）：376-382.
17	蔡联炳，张梅妞.国产赖草属的叶表皮特征与组群划分［J］.植物研究，2005，25（4）：400-405.
	Cai L B，Zhang M N.Leaf epidermal characteristics and classification of sections of Leymus from China［J］.Bulletin of Botanical Research，2005，25（4）：400-405.
18	姚雪莹，陈志祥，王奇志.变豆菜属11种植物的叶表皮微形态特征研究［J］.植物研究，2019，39（5）：683-691.
	Yao X Y，Chen Z X，Wang Q Z.Leaf epidermal micromorphological characters of 11 species of Sanicula L.［J］.Bulletin of Botanical Research，2019，39（5）：683-691.
19	Bublyk O M，Andreev I O，Kalendar R N，et al.Efficiency of different PCR-based marker systems for assessment of Iris pumila genetic diversity［J］.Biologia，2013，68（4）：613-620.
20	Li H，Madden J L，Potts B M.Variation in leaf waxes of the Tasmanian Eucalyptus species-Ⅰ.Subgenus Symphyomyrtus［J］.Biochemical Systematics and Ecology，1997，25（7）：631-657.
21	Kong H Z.Comparative morphology of leaf epidermis in the Chloranthaceae［J］.Botanical Journal of the Linnean Society，2001，136（3）：279-294.
22	雒宏佳，刘亚斌，常朝阳.29种中国野豌豆属植物叶表皮微形态特征及其系统学意义［J］.西北植物学报，2015，35（1）：76-88.
	Luo H J，Liu Y B，Chang Z Y.Leaf epidermal features of 29 species of the genus Vicia L. from China［J］.Acta Botanica Boreali-Occidentalia Sinica，2015，35（1）：76-88.
23	孙同兴，董运秋，侯学良.中国罗汉松属叶角质层微形态结构及其分类意义［J］.植物研究，2019，39（2）：169-180.
	Sun T X，Dong Y Q，Hou X L.Cuticle micromorphology of Podocarpus from china and its taxonomic significance［J］.Bulletin of Botanical Research，2019，39（2）：169-180.
24	Ghahremaninejad F，Khalili Z，Maassoumi A A，et al.Leaf epidermal features of Salix species（Salicaceae） and their systematic significance［J］.American Journal of Botany，2012，99（4）：769-777.
25	王东超，吴杰，杨永红，等.叶表皮蜡质纹饰特征的种内变异—以青藏高原地区的紫花针茅和小叶棘豆为例［J］.植物分类与资源学报，2013，35（3）：348-354.
	Wang D C，Wu J，Yang Y H，et al.Intraspecific variation of leaf epidermal cuticle waxes under scanning electronic microscope：Stipa purpurea and Oxytropis microphylla from the Qinghai-Tibet plateau［J］.Plant Diversity and Resources，2013，35（3）：348-354.
26	Shi J H，Li L Q.Leaf epidermal feature in Clematis（Ranunculaceae） with reference to its systematic significance［J］.Acta Botanica Sinica，2003，45（3）：257-268.
27	叶泉清，薛跃规.淡黄金花茶归并种的形态特征比较及分类探讨［J］.中山大学学报：自然科学版，2013，52（3）：103-111.
	Ye Q Q，Xue Y G.Comparisions of morphological characters of some Camellia species which were reduced to Camellia flavida H.T.Chang and discussion on their taxonomic status［J］.Acta Scientiarum Naturalium Universitatis Sunyatseni，2013，52（3）：103-111.
28	Karbulková J，Schreiber L，Macek P，et al.Differences between water permeability of astomatous and stomatous cuticular membranes：effects of air humidity in two species of contrasting drought-resistance strategy［J］.Journal of Experimental Botany，2008，59（14）：3987-3995.
29	Ebrahim S，Usha K，Singh B.Plant architectural traits and their role in defense mechanism against malformation in mango（Mangifera Indica L.）［J］.Scientia Horticulturae，2012，139：25-31.
30	朱栗琼，徐艳霞，招礼军，等.喀斯特地区莎叶兰的解剖构造及其环境适应性［J］.广西植物，2016，36（10）：1179-1185，1164.
	Zhu L Q，Xu Y X，Zhao L J，et al.Anatomical structure and environmental adaptability of Cymbidium cyperifolium in Karst area［J］.Guihaia，2016，36（10）：1179-1185，1164.

种类 Species	气孔器位置 Position	气孔外拱盖外缘角质层 outer margin of cuticle	气孔外拱盖角质膜两侧 Both sides of the Cuticular membrane	气孔外拱盖表面 Surface	气孔外拱盖内缘 Inner margin
IM	平Flush	1层，不完全环形 One layer，incomplete annular	平滑Smooth	近平滑 Nearly smooth	较平直Straight
CH	平Flush	多层，不明显环形 Multilayer，unobvious annular	平滑Smooth	近平滑 Nearly smooth	浅波状Sinuolate
PT	平Flush	1层，环形 One layer，annular	波状Sinuous	近平滑 Nearly smooth	浅波状Sinuolate
LI	下陷Sunken	1层，环形 One layer，annular	平滑Smooth	近平滑 Nearly smooth	浅波状Sinuolate
LO	平Flush	1层，环形 One layer，annular	波状Sinuous	近平滑 Nearly smooth	浅波状Sinuolate
PE	下陷Sunken	2层，环形 Two layer，annular	平滑Smooth	粗糙 Rough	浅波状Sinuolate

种类 Species	气孔长度 length of stomata （μm）	气孔宽度 Width of stomata （μm）	气孔密度 Density of stomata （N·mm^-2）	气孔指数 Index of stomata （%）	上表皮密度 Density of adaxial epidermis（N·mm^-2）	下表皮密度 Density of abaxial epidermis（N·mm^-2）
IM	14.60±0.23a	8.64±0.11b	232.97±4.69a	16.14	3 053.74±54.18b	1 210.76±28.41b
CH	15.57±0.16a	9.38±0.14a	246.57±4.47a	13.72	3 266.90±48.09a	1 550.87±28.27a
PT	15.27±0.32a	9.52±0.13a	195.56±5.91c	14.18	2 488.60±42.15c	1 183.55±25.70b
LI	14.62±0.19a	9.85±0.18a	149.65±5.00e	15.71	2 248.07±34.41d	802.64±21.73c
LO	15.51±0.36a	9.64±0.20a	176.00±7.17d	12.98	2 166.37±31.64d	1 180.15±27.10b
PE	12.28±0.34b	8.14±0.25c	215.11±7.70b	20.93	2 002.77±31.68e	812.84±18.62c

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