Bulletin of Botanical Research ›› 2022, Vol. 42 ›› Issue (6): 1023-1032.doi: 10.7525/j.issn.1673-5102.2022.06.012
• Molecular biology • Previous Articles Next Articles
Huafeng CHEN1, Longjun DAI2, Mingyang LIU2, Bingbing GUO2, Hong YANG2, Lifeng WANG2()
Received:
2022-06-17
Online:
2022-11-20
Published:
2022-11-22
Contact:
Lifeng WANG
E-mail:lfwang@catas.cn
About author:
CHEN Huafeng(1978—),male,postgraduate,major in plant metabolism and physiological ecology.
Supported by:
CLC Number:
Huafeng CHEN, Longjun DAI, Mingyang LIU, Bingbing GUO, Hong YANG, Lifeng WANG. Stress Tolerance Functional Analysis of the High Expression Heat Shock Protein HbHSP90.4 Gene from the Latex of Hevea brasiliensis[J]. Bulletin of Botanical Research, 2022, 42(6): 1023-1032.
Add to citation manager EndNote|Ris|BibTeX
URL: https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2022.06.012
Table 1
Bioinformatics online analysis tools
名称 Name | 网址 Website |
---|---|
NCBI ORF Finder | www.ncbi.nlm.nih.gov/orffinder/ |
NCBI smart BLAST | blast.ncbi.nlm.nih.gov/Blast.cgi |
ExPASy ProtParam tool | web.expasy.org/protparam/ |
TMHMM Server v.2.0 | www.cbs.dtu.dk/services/TMHMM-2.0/ |
DeepLoc1.0 | www.cbs.dtu.dk/services/DeepLoc/ |
SignalP-5.0 Server | services.healthtech.dtu.dk/service.php?SignalP-5.0 |
SWISS-MODEL | swissmodel.expasy.org/ |
PSIPRED Workbench | bioinf.cs.ucl.ac.uk/psipred/ |
Table 2
Primers of HbHSP90.4 gene
引物名称 Primer name | 序列 Sequence(5′→3′) | 用途 Application |
---|---|---|
HbHSP90.4-F | ATCTATTGAACGGGAGA | 编码区PCR扩增 ORF PCR amplification |
HbHSP90.4-R | GCACTAAGGGCCAAG | |
HbHSP90.4-QF | GGACGGGAATGTAAAGCAAAC | 荧光定量 Fluorescence quantitative |
HbHSP90.4-QR | GCTGTAGATACGGGAAGCAAAA | |
HbActin-F | GATGTGGATATCAGGAAGGA | 内参基因 Reference gene |
HbActin-R | CATACTGCTTGGAGCAAGA | |
HbHSP90.4-MF | acgggggacgagctc | 表达载体构建 Construction of expression vector |
HbHSP90.4-MR | catgtcgactctaga |
1 | 唐朝荣.橡胶树产胶生物学研究进展[J].热带作物学报,2020,41(10):1958-1967. |
TANG C R.Research progress on biology of rubber production in rubber tree[J].Chinese Journal of Tropical Crops,2020,41(10):1958-1967. | |
2 | WARREN-THOMAS E M, EDWARDS D P, BEBBER D P,et al.Protecting tropical forests from the rapid expansion of rubber using carbon payments[J].Nature Communications,2018,9:911. |
3 | LI X, LIU Y H, HE Q G,et al.A candidate secreted effector protein of rubber tree powdery mildew fungus contributes to infection by regulating plant ABA biosynthesis[J].Frontiers in Microbiology,2020,11:591387. |
4 | DOS SANTOS J O, DE OLIVEIRA L E M, DE SOUZA T,et al.Physiological mechanisms responsible for tolerance to,and recuperation from,drought conditions in four different rubber clones[J].Industrial Crops and Products,2019,141:111714. |
5 | TAIPALE M, JAROSZ D F, LINDQUIST S.HSP90 at the hub of protein homeostasis:emerging mechanistic insights[J].Nature Reviews Molecular Cell Biology,2010,11(7):515-528. |
6 | KRISHNA P, GLOOR G.The Hsp90 family of proteins in Arabidopsis thaliana [J].Cell Stress & Chaperones,2001,6(3):238-246. |
7 | WANG W X, VINOCUR B, SHOSEYOV O,et al.Role of plant heat-shock proteins and molecular chaperones in the abiotic stress response[J].Trends in Plant Science,2004,9(5):244-252. |
8 | SABLE A, RAI K M, CHOUDHARY A,et al.Inhibition of Heat Shock proteins HSP90 and HSP70 induce oxidative stress,suppressing cotton fiber development[J].Scientific Reports,2018,8:3620. |
9 | YAMADA K, FUKAO Y, HAYASHI M,et al.Cytosolic HSP90 regulates the heat shock response that is responsible for heat acclimation in Arabidopsis thaliana [J].Journal of Biological Chemistry,2007,282(52):37794-37804. |
10 | WANG T, LI J X, SHEN Q H.Regulation of NLR stability in plant immunity[J].Frontiers of Agricultural Science and Engineering,2019,6(2):97-104. |
11 | MILIONI D, HATZOPOULOS P.Genomic organization of hsp90 gene family in Arabidopsis [J].Plant Molecular Biology,1997,35(6):955-961. |
12 | SONG H M, ZHAO R M, FAN P X,et al.Overexpression of AtHsp90.2,AtHsp90.5 and AtHsp90.7 in Arabidopsis thaliana enhances plant sensitivity to salt and drought stresses[J].Planta,2009,229(4):955-964. |
13 | LACHOWIEC J, LEMUS T, BORENSTEIN E,et al.Hsp90 promotes kinase evolution[J].Molecular Biology and Evolution,2015,32(1):91-99. |
14 | ZHAO R M, HOURY W A.Hsp90:a chaperone for protein folding and gene regulation[J].Biochemistry and Cell Biology-Biochimie et Biologie Cellulaire,2005,83(6):703-710. |
15 | LIU Y L, BURCH-SMITH T, SCHIFF M,et al.Molecular chaperone Hsp90 associates with resistance protein N and its signaling proteins SGT1 and Rar1 to modulate an innate immune response in plants[J].Journal of Biological Chemistry,2004,279(3):2101-2108. |
16 | TAKAHASHI A, CASAIS C, ICHIMURA K,et al.HSP90 interacts with RAR1 and SGT1 and is essential for RPS2-mediated disease resistance in Arabidopsis [J].Proceedings of the National Academy of Sciences of the United States of America,2003,100(20):11777-11782. |
17 | PRASAD B D, GOEL S, KRISHNA P.In silico identification of carboxylate clamp type tetratricopeptide repeat proteins in Arabidopsis and rice as putative co-chaperones of Hsp90/Hsp70[J].PLoS One,2010,5(9):e12761. |
18 | WANG L F.Physiological and molecular responses to drought stress in rubber tree(Hevea brasiliensis Muell.Arg.) [J].Plant Physiology and Biochemistry,2014,83:243-249. |
19 | QIN B, ZHENG F C, ZHANG Y.Molecular cloning and characterization of a Mlo gene in rubber tree (Hevea brasiliensis)[J].Journal of Plant Physiology,2015,175:78-85. |
20 | 柯宇航,刘明洋,王燕,等.橡胶树HbRPM1-3基因克隆及其应答白粉菌侵染的功能分析[J].植物生理学报,2021,57(11):2167-2178. |
KE Y H, LIU M Y, WANG Y,et al.Cloning of HbRPM1-3 gene from Hevea brasiliensis and functional analysis of its response to powdery mildew infection[J].Plant Physiology Journal,2021,57(11):2167-2178. | |
21 | KUMAR S, STECHER G, LI M,et al.MEGA X:molecular evolutionary genetics analysis across computing platforms[J].Molecular Biology and Evolution,2018,35(6):1547-1549. |
22 | SCHMITTGEN T D, LIVAK K J.Analyzing real-time PCR data by the comparative CT method[J].Nature Protocols,2008,3(6):1101-1108. |
23 | DUAN C F,RIO M, LECLERCQ J,et al.Gene expression pattern in response to wounding,methyl jasmonate and ethylene in the bark of Hevea brasiliensis [J].Tree Physiology,2010,30(10):1349-1359. |
24 | SAMAKOVLI D, TICHÁ T, VAVRDOVÁ T,et al.YODA-HSP90 module regulates phosphorylation-dependent inactivation of SPEECHLESS to control stomatal development under acute heat stress in Arabidopsis [J].Molecular Plant,2020,13(4):612-633. |
25 | ZHANG J, LI J B, LIU B B,et al.Genome-wide analysis of the Populus Hsp90 gene family reveals differential expression patterns,localization,and heat stress responses[J].BMC Genomics,2013,14:532. |
26 | THIRUMALAIKUMAR V P, GORKA M, SCHULZ K,et al.Selective autophagy regulates heat stress memory in Arabidopsis by NBR1-mediated targeting of HSP90.1 and ROF1[J].Autophagy,2021,17(9):2184-2199. |
27 | LI D X, WANG M W, ZHANG T P,et al.Glycinebetaine mitigated the photoinhibition of photosystem II at high temperature in transgenic tomato plants[J].Photosynthesis Research,2021,147(3):301-315. |
28 | SONG Z P, PAN F L, YANG C,et al.Genome-wide identification and expression analysis of HSP90 gene family in Nicotiana tabacum [J].BMC Genetics,2019,20(1):35. |
29 | 王立丰,安锋,王纪坤,等.不同旱害级别对开割和未开割橡胶树形态和生理特性的影响[J].热带作物学报,2017,38(10):1777-1783. |
WANG L F, AN F, WANG J K,et al.Effects of different drought grades on the morphological and physiological characterizations of tapping tree and virgin tree of Hevea brasiliensis [J].Chinese Journal of Tropical Crops,2017,38(10):1777-1783. | |
30 | XIANG J H, CHEN X B, HU W,et al.Overexpressing heat-shock protein OsHSP50.2 improves drought tolerance in rice[J].Plant Cell Reports,2018,37(11):1585-1595. |
31 | ZHANG M, SHEN Z W, MENG G Q,et al.Genome-wide analysis of the Brachypodium distachyon(L.) P.Beauv.Hsp90 gene family reveals molecular evolution and expression profiling under drought and salt stresses[J].PLoS One,2017,12(12):e0189187. |
32 | LIYANAGE K K, KHAN S, BROOKS S,et al.Morpho-molecular characterization of two Ampelomyces spp.(Pleosporales) strains mycoparasites of powdery mildew of Hevea brasiliensis [J].Frontiers in Microbiology,2018,9:12. |
33 | HEIN I, BARCISZEWSKA-PACAK M, HRUBIKOVA K,et al.Virus-induced gene silencing-based functional characterization of genes associated with powdery mildew resistance in barley[J].Plant Physiology,2005,138(4):2155-2164. |
34 | LI N, HAN X, FENG D,et al.Signaling crosstalk between salicylic acid and ethylene/jasmonate in plant defense:Do we understand what they are whispering?[J].International Journal of Molecular Sciences,2019,20(3):671. |
35 | ZHANG X C, MILLET Y A, CHENG Z Y,et al.Jasmonate signalling in Arabidopsis involves SGT1b-HSP70-HSP90 chaperone complexes[J].Nature Plants,2015,1:15049. |
36 | CHRESTIN H.Biochemical aspects of bark dryness induced by overstimulation of rubber trees with ethrel[M]//Physiology of Rubber Tree Latex.Florida:CRC Press,1989:431-441. |
[1] | Zhanmin ZHENG, Yubing SHANG, Guangbo ZHOU, Di XIAO, Yi LIU, Xiangling YOU. Genetic Transformation and Function Analysis of PsnHB13 and PsnHB15 of Populus simonii × Populus nigra [J]. Bulletin of Botanical Research, 2023, 43(3): 340-350. |
[2] | Shixian LIAO, Yuting WANG, Liben DONG, Yongmei GU, Fenglin JIA, Tingbo JIANG, Boru ZHOU. Function Analysis of the Transcription Factor PsnbZIP1 of Populus simonii×P. nigra in Response to Salt Stress [J]. Bulletin of Botanical Research, 2023, 43(2): 288-299. |
[3] | Senyao LIU, Fenglin JIA, Qing GUO, Gaofeng FAN, Boru ZHOU, Tingbo JIANG. Response Analysis of Transcription Factor PsnbHLH162 Gene in Populus simonii × P. nigra under Salt Stress and Low Temperature Stress [J]. Bulletin of Botanical Research, 2023, 43(2): 300-310. |
[4] | Anying HUANG, Dean XIA, Yang ZHANG, Dongchen NA, Qing YAN, Zhigang WEI. Cloning and Drought Tolerance Expression Analysis of PtrWRKY51 Gene in Populus trichocarpa [J]. Bulletin of Botanical Research, 2022, 42(6): 1005-1013. |
[5] | Denggao LI, Rui LIN, Qinghui MU, Na ZHOU, Yanru ZHANG, Wei BAI. Identification and Analysis of the Potato StCRKs Gene Family and Expression Patterns in Response to Stress Signals [J]. Bulletin of Botanical Research, 2022, 42(6): 1033-1043. |
[6] | Mingyang LIU, Huaxing XIAO, Lifeng WANG, Xiaoxu LIANG, Yu ZHANG, Meng WANG. Cloning and Functional Analysis of Heat Shock Protein HbHSP90.8-1 from Hevea brasiliensis Müll. Arg. [J]. Bulletin of Botanical Research, 2022, 42(5): 811-820. |
[7] | He CHENG, Shuanghui TIAN, Yang ZHANG, Cong LIU, De’an XIA, Zhigang WEI. Genome-wide Identification and Expression Analysis of nsLTP Gene Family in Populus trichocarpa [J]. Bulletin of Botanical Research, 2022, 42(3): 412-423. |
[8] | Qian Sun, Yuhang Wu, Yaxuan Zhang, Jingdan Cao, Jingjing Shi, Chao Wang. Bioinformatic Analysis and Expression Pattern of LTP Family Genes in Populus davidiana × P. alba var. pyramidalis [J]. Bulletin of Botanical Research, 2022, 42(2): 211-223. |
[9] | Yuning Yang, Hao Dong, Shiwei Dong, Nairui Wang, Yue Song, Hanguo Zhang, Shujuan Li. Cloning and Expression Analysis of Transcription Factor LobHLH34 from Larix olgensis [J]. Bulletin of Botanical Research, 2022, 42(1): 112-120. |
[10] | Shuang Ma, Boya Wang, Ying Cao, Shanglian Hu, Zhimin Gao. Identification and Expression Analysis of Expansin Genes in Moso Bamboo (Phyllostachys edulis) [J]. Bulletin of Botanical Research, 2022, 42(1): 29-38. |
[11] | Jiaming Zhao, Erqin Fan, Yi Liu, Zhi Wang, Junhui Wang, Guanzheng Qu. Cloning and Bioinformatics Analysis of CbuATX1,CbuATX1-like and CbuATX2 Genes from Catalpa bungei [J]. Bulletin of Botanical Research, 2022, 42(1): 47-61. |
[12] | Xin-Tong JI, Lei YU, Ya-Guang ZHAN. Cloning and Expression Analysis of BRASSINAZOLE RESISTANTANT1 (BZR1) Gene from Fraxinus mandshurica [J]. Bulletin of Botanical Research, 2021, 41(5): 744-752. |
[13] | Hua-Feng CHEN, Yu-Qing TANG, Ya-Jie PAN, Xiao-Rui GUO. Progress on the Metabolic Basis and Regulation Mechanism of Fruit Flavor [J]. Bulletin of Botanical Research, 2021, 41(3): 474-480. |
[14] | Shu-Ping PENG, Cheng-Ming DONG, Yun-Hao ZHU. Cloning and Expression Analysis of Two Key Genes of Jasmonic Acid Synthesis in Response to Endophytic Infection from Rehmannia glutinosa [J]. Bulletin of Botanical Research, 2021, 41(2): 294-301. |
[15] | Xiao-Xiao WANG, Bi QIN, Yu-Shuang YANG, Qiu-Hai NIE, Ji-Chuan ZHANG, Shi-Zhong LIU. Cloning and Expression Analysis of E2 Ubiquitin-conjugating Enzyme Gene TkUBC2 in Taraxacum kok-saghyz Rodin [J]. Bulletin of Botanical Research, 2021, 41(1): 98-106. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||