Variation of Lignin Content and Association Analysis of FmPAL Nucleotide Polymorphism in Progenies of Interspecific Hybrids of Fraxinus

doi:10.7525/j.issn.1673-5102.2022.02.004

Abstract

Abstract:

To improve materials for genetic breeding of Fraxinus mandshurica， the correlation among three hybrid combinations of Fraxinus mandshurica × F. rhynchophylla， F. mandshurica × F. sogdiana and F. mandshurica × F. velutina， and the genetic variation of lignin content and FmPAL nucleotide polymorphism in free-pollination offspring of female parent were analyzed respectively. One-year-old branches of Fraxinus mandshurica with 71 species of interspecies hybrid combinations in Maoer Mountain Forest Farm of Northeast Forestry University were used as materials， the genetic variation regularity of lignin was investigated， the differences in lignin content and variation parameters and mean values were analyzed， and joint analysis on the molecular level of lignin and its synthesis-related gene PAL were carried out. The result showed that the lignin content of the hybrid combinations of Fraxinus was extremely significant， and it was significantly different from the female parent free-pollinated offspring of F. mandshurica. The lignin content of most hybrid combinations of F. mandshurica was higher than that of the female parent F. mandshurica. The results showed that the haploid diversity value of the PAL gene of the F. mandshurica population reached 0.999 7， which was a high level. It confirmed that the FmPAL gene had a high variation. There were 3 SNP sites significantly related to the lignin content. There were significant differences in the lignin content of the cross combinations of F. mandshurica. The SNP sites in the FmPAL gene was significantly related to the lignin content.

Key words: Fraxinus, Fraxinus mandshurica, Lignin, Genetic variation, FmPAL, SNP

CLC Number:

Q786

Yibo Yin, Jixiang Li, Yingjie Guo, Ziting Lu, Ying Xiao, Hualing Liu, Yaguang Zhan, Fansuo Zeng. Variation of Lignin Content and Association Analysis of FmPAL Nucleotide Polymorphism in Progenies of Interspecific Hybrids of Fraxinus[J]. Bulletin of Botanical Research, 2022, 42(2): 191-199.

Figures/Tables 6

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水曲柳×大叶白蜡 F. mandshurica × F. rhynchophylla		水曲柳×小叶白蜡 F. mandshurica × F. sogdiana		水曲柳×绒毛白蜡 F. mandshurica × F. velutina		母本自由授粉子代 Free pollination offspring
杂交组合 Cross combination	木质素含量 Lignin content /%	杂交组合 Cross combination	木质素含量 Lignin content /%	杂交组合 Cross combination	木质素含量 Lignin content /%	母本对照 Maternal control	木质素含量 Lignin content /%
70	32.96±5.25	129	35.29±1.71	7	39.79±7.15	135	37.20±3.73
131	32.94±3.24	88	33.50±1.15	63	32.49±2.59	M1	35.50±5.36
94	32.88±3.19	31	32.87±0.88	48	32.19±4.55	17	31.46±0.52
69	31.54±2.43	76	31.25±1.24	66	31.34±2.20	23	31.25±1.63
13	30.50±0.90	65	30.28±0.82	116	31.20±0.37	M6	29.80±2.53
110	30.16±0.84	58	29.71±4.17	55	29.50±5.36	42	28.41±3.08
12	29.67±2.71	15	28.87±1.62	104	28.54±2.91	M16	28.38±2.82
43	28.73±1.85	138	28.83±2.27	56	28.47±1.24	127	28.20±1.17
34	28.03±2.99	87	28.71±3.11	44	28.30±3.91	M11	26.39±0.87
32	27.96±1.88	62	28.24±0.84	54	28.24±1.43	52	25.77±3.40
100	27.64±5.91	123	27.43±2.53	103	28.17±4.82	M3	24.15±3.46
45	27.64±0.64	60	27.14±0.90	28	27.99±2.81	98	23.85±12.25
134	27.43±0.53	89	25.38±0.58	67	27.60±1.88	M13	23.81±6.13
86	27.26±9.25	24	25.05±1.65	111	27.22±0.89	30	23.81±0.74
72	27.17±2.36	125	24.68±1.46	5	26.97±1.03	M10	15.20±3.26
128	26.71±1.12	75	21.00±9.40	112	26.53±1.59	92	14.56±1.52
36	26.36±6.14	90	19.49±7.62	46	26.30±2.23
49	26.23±2.48	22	19.19±1.09	3	26.25±2.67
10	26.08±2.21			107	25.94±2.82
126	26.08±0.84			64	25.30±4.53
71	25.38±4.51			4	25.20±3.01
73	24.03±3.68			39	25.19±1.01
115	21.85±1.59			41	25.09±2.16
74	19.66±1.19			78	25.00±9.76
9	17.92±2.23			117	24.72±2.47
				91	22.96±2.16
				51	22.65±1.67
				102	15.67±5.39

组合 combination	自由度 df	均方 Mean square	F	均值 Mean /%	变幅 Luffing /%	变异系数 PCV /%	遗传力 Heritability /h²
水曲柳×大叶白腊 F. mandshurica × F. rhynchophylla	24	50.187	2.915	27.31	17.28~36.07	17.27	0.657
水曲柳×小叶白腊 F. mandshurica × F. sogdiana	17	104.196	4.028	26.50	11.98~35.29	24.43	0.751
水曲柳×绒毛白腊 F. mandshurica × F. velutina	27	87.293	5.013	27.38	11.80~39.79	19.01	0.801
母本自由授粉子代 Free pollination offspring	15	192.045	9.534	25.53	11.59~37.20	28.03	0.895

组合 combination	自由度 df	均方 Mean square	F
水曲柳×大叶白腊 F. mandshurica × F. rhynchophylla	52	52.642	7.303
水曲柳×小叶白腊 F. mandshurica × F. sogdiana	27	117.815	22.160
水曲柳×绒毛白腊 F. mandshurica ×F. velutina	65	74.145	12.007
母本自由授粉子代 Free pollination offspring	27	145.719	25.828

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