Differences in Seed Dormancy Among Different Clones of Fraxinus mandshurica

doi:10.7525/j.issn.1673-5102.2024.05.008

Abstract

Abstract:

Seeds of 18 F. mandshurica clones were used as materials， the germination performances of clones at suitable temperatures after different stratification treatments were compared， and the relationships between germination performances and seed embryo growth changes were analyzed. The results showed that the germination performances of seeds were different among F. mandshurica clones after different stratification treatments. Seed dormancy of clones 1， 2 and 6 was shallow and could be broken after variable temperature stratification treatments for a short time（a total 18 weeks）， which the germination rate of clones 2 and 6 reached more than 75%. Seed dormancy of clones 13， 14， 15 and 16 were broken by 20-week stratification treatment （cold temperature extended for two weeks）， and clones 4， 8， 11 and 12 by 20-week stratification treatment （warm temperature extended for two weeks）. Seeds dormancy of clones 5 and 18 was deep-degree， it could be broken by extending the stratification（warm and cold temperature） time to 22 weeks. Seed dormancy of clones 3， 7， 9， 10 and 17 could be broken by stratification treatment for 20 weeks， without significant difference in the dormancy between seeds treated by extending warm and cold stratification time. The changes of embryo length， embryo rate and embryo mass ratio in the process of stratification were different in different clones， and relative extension time（two weeks） of cold temperature stratification in clones 6， 7 and 11 was conducive to embryo growth， and extension time（two weeks） of warm temperature stratification in clones 2， 5 and 17 was conducive to embryo growth. Differences in seed dormancy of different clones could not be accurately judged by changes in seed embryo growth during the stratification process， which should be judged mainly by the germination performances. The results could provide a reference for the selection of mother clones with shallow seed dormancy in production practice.

Key words: Fraxinus mandshurica, seeds, clones, breaking dormancy, embryo growth

CLC Number:

Q945.6

Ting LIU, Mingyue LI, Meiru ZHU, Hao XIN, Bowen DONG, Peng ZHANG. Differences in Seed Dormancy Among Different Clones of Fraxinus mandshurica[J]. Bulletin of Botanical Research, 2024, 44(5): 711-720.

Figures/Tables 8

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处理 Treatment	暖温层积时长 Warm temperature stratification time/week	低温层积时长 Cold temperature Stratification time/week	总时长 Total duration/week	描述 Description
S1	10	8	18	较短时间层积处理 Short-time stratification treatment
S2	10	10	20	相对延长低温时间的中等时间层积处理 Medium-time stratification treatment with extension at time of cold temperature treatment
S3	12	8	20	相对延长暖温时间的中等时间层积处理 Medium-time stratification treatment with extension at time of warm temperature treatment
S4	12	10	22	较长时间层积处理 Long-time stratification treatment

无性系 Clones	发芽率 Germination rate/%
无性系 Clones	S1	S2	S3	S4
1	56.25±6.33Abcd	61.25±9.44Adef	42.50±5.95Ag	59.50±3.71Agh
2	76.00±4.32Aa	70.00±3.54Abcde	77.50±4.78Aab	80.00±4.56Aabcd
3	60.00±3.65Babc	68.75±4.27Acde	67.50±4.79Aabcdef	77.75±1.11Abcde
4	19.75±8.70Bfg	32.50±2.50Bg	56.25±7.11Acdefg	68.50±1.26Adefgh
5	39.25±11.00Bdef	43.75±3.15Bfg	63.75±2.40Bbcdef	73.75±2.43Abcdef
6	75.00±3.79Aab	78.50±1.71Aabcd	71.50±1.76Aabcd	73.75±2.75Abcdef
7	13.75±4.16Bg	45.25±7.36Afg	53.75±9.66Adefg	57.50±4.79Ah
8	38.75±8.54Cdef	55.00±6.12BCef	73.75±6.56ABabc	81.25±2.78Aabc
9	36.00±5.16Bef	56.25±2.39Aef	51.25±4.27Aefg	63.75±3.75Afgh
10	33.00±6.61Bfg	55.00±9.35Aef	52.50±5.20ABefg	66.00±2.61Aefgh
11	31.25±2.52Cfg	46.25±4.73Bfg	68.75±4.27Aabcde	77.50±3.62Abcde
12	54.50±6.81Ccde	68.75±8.00BCcde	82.50±4.33ABa	90.50±3.97Aa
13	73.25±1.92Babc	91.25±3.15Aa	61.25±4.27Cbcdef	81.75±3.90ABab
14	66.00±6.63Babc	81.50±3.89Aabcd	42.50±3.23Cg	69.00±4.20ABcdefgh
15	38.25±3.46Cdef	73.75±3.15Aabcde	52.50±4.33Befg	71.25±4.33Abcdefg
16	37.00±3.42Cdef	87.50±2.50Aabc	61.25±5.15Bbcdef	79.00±4.95Aabcd
17	28.25±9.24Bfg	60.00±1.20Aef	56.25±7.18Acdefg	75.50±5.38Abcde
18	36.25±3.65Bef	57.50±10.90ABef	50.00±7.07ABfg	72.75±4.40Abcdef

无性系 Clones	发芽指数 Germination index
无性系 Clones	S1	S2	S3	S4
1	2.98±0.73Aabcd	3.38±0.60Aabcd	0.66±0.12Bf	0.74±0.09Bi
2	3.88±0.62Aab	2.96±0.37Abcde	1.64±0.27Bcde	3.20±0.25Aabc
3	2.25±0.23Bcdef	3.25±0.36Abcd	1.53±0.04Bcde	1.62±0.20Bfghi
4	0.62±0.36Ahi	1.18±0.31Ag	1.19±0.12Aef	1.03±0.13Ahi
5	1.39±0.46Afhi	1.15±0.23Ag	1.28±0.10Aef	1.83±0.28Aefgh
6	2.61±0.32Abcde	2.85±0.56Abcde	0.69±0.12Bf	1.33±0.24Bghi
7	0.32±0.08Chi	1.60±0.55Befg	2.11±0.36ABcd	2.99±0.38Aabcd
8	1.78±0.30Bdef	2.69±0.50ABcdef	3.30±0.51Aa	3.48±0.24Aab
9	1.42±0.27Bfhi	2.71±0.33Acdef	1.64±0.32Bcde	3.01±0.32Aabcd
10	1.16±0.25Bhi	2.10±0.32Adefg	1.10±0.13Bef	0.98±0.09Bhi
11	0.78±0.17Bhi	1.31±0.33ABfg	2.15±0.27Abcd	1.58±0.40ABghi
12	2.84±0.57ABabcde	1.50±0.26Cefg	2.18±0.10BCbc	3.76±0.27Aa
13	4.06±0.29Ba	5.52±0.40Aa	2.78±0.12Bab	2.66±0.46Bbcde
14	3.15±0.46Aabc	4.09±0.96Aabc	1.10±0.07Bef	2.44±0.47ABcdef
15	2.61±0.66Bbcde	4.26±0.44Aab	1.46±0.16Bde	2.13±0.28Bdefg
16	1.63±0.29Befhi	2.58±0.04Adefg	1.15±0.09Bef	1.61±0.28Bfghi
17	0.81±0.21Ahi	1.23±0.10Afg	1.13±0.25Aef	1.27±0.10Aghi
18	1.42±0.14BCfhi	2.30±0.60ABfg	1.23±0.12Cef	2.50±0.14Acdef

无性系 Clones	发芽时间 Germination time/d
无性系 Clones	S1	S2	S3	S4
1	7.22±0.61Cde	5.89±0.24Cf	14.26±0.87Aa	9.44±0.82Bbcd
2	8.77±0.20Bcde	11.62±1.18Aab	11.41±0.58Abcd	6.52±0.29Cfgh
3	10.46±1.10Abcde	7.01±0.11Bef	10.21±0.88Acdefg	7.72±0.43Bdefg
4	14.43±3.57Aab	9.73±0.71Abcd	12.21±0.42Abc	12.89±0.43Aa
5	13.24±1.72Aabc	11.18±0.99Aabc	11.22±0.59Abcde	9.47±0.79Abcd
6	10.71±0.58Babcde	9.14±0.58BCcde	14.18±0.63Aa	8.74±0.37Ccde
7	11.94±2.31Aabcd	8.25±0.91Ade	6.62±0.75Bi	5.57±0.57Bgh
8	7.08±0.79ABe	8.63±1.36Ade	6.68±0.26ABi	4.81±0.37Bh
9	8.80±1.21ABcde	8.92±0.52ABcde	9.10±0.85Afgh	5.81±0.44Bgh
10	9.61±0.60ABcde	8.25±0.63Bde	11.00±0.64Abcdef	9.36±1.22ABbcd
11	15.17±1.85Aa	12.58±0.93Aa	8.11±0.55Bghi	6.44±1.07Bfgh
12	7.41±0.88Bde	11.21±0.33Aabc	8.92±0.36Bfgh	5.31±0.51Ch
13	6.40±0.74Ae	4.47±0.23Bf	7.29±0.46Ahi	6.96±0.38Aefgh
14	7.40±0.22ABde	6.83±0.37Bef	8.53±0.54Aghi	5.18±0.68Ch
15	6.66±1.08Ae	7.07±0.77Aef	9.22±0.89Aefgh	8.06±0.57Adef
16	9.40±1.56Acde	9.68±0.48Abcd	11.71±0.27Abcd	10.21±0.81Abc
17	8.59±0.92Bcde	11.04±0.17ABabc	12.85±0.95Aab	11.03±0.74ABab
18	9.39±1.20Acde	9.50±0.63Abcd	9.74±0.69Adefg	6.53±0.61Bfgh

无性系 Clones	胚长 Embryo length/mm	胚率 Ratio of embryo length to kernel length/%	胚质量比 Ratio of embryo dry weight to kernel dry weight/%
1	0.36±0.15fg	0.97±0.35e	3.92±1.31bcdef
2	1.43±0.36defg	4.99±0.71bcde	5.74±0.45abcde
3	1.35±0.58defg	3.10±2.08cde	6.97±1.50abc
4	1.17±0.57defg	6.25±4.10bcde	3.50±0.68cdef
5	1.87±0.29de	6.50±3.74bcde	5.04±1.31bcdef
6	1.36±0.29defg	4.76±1.92bcde	2.95±3.25cdef
7	2.04±0.44d	5.71±5.45bcde	1.22±1.39f
8	1.68±0.07de	4.62±1.48bcde	7.62±0.62ab
9	2.51±0.54bc	11.02±1.46bcd	4.66±0.81bcdef
10	1.85±0.18de	5.63±2.47bcde	2.80±1.26def
11	0.18±0.45g	1.00±0.34e	3.23±0.33cdef
12	2.00±0.40d	8.13±3.80bcde	3.33±0.92cdef
13	3.77±0.30ab	16.10±0.27a	8.20±0.20a
14	4.01±0.27a	16.57±2.80a	1.83±0.41ef
15	2.40±0.15cd	9.44±0.45bcde	5.30±0.85bcdef
16	3.58±0.94abc	12.88±5.68ab	6.30±0.89abcd
17	0.45±0.62efg	2.81±1.60de	4.66±1.14bcdef
18	1.67±0.24de	12.47±1.99bc	5.69±0.85abcde