Welcome to Bulletin of Botanical Research! Today is Share:

Bulletin of Botanical Research ›› 2022, Vol. 42 ›› Issue (2): 184-190.doi: 10.7525/j.issn.1673-5102.2022.02.003

• Genetic and Breeding • Previous Articles     Next Articles

Rapid Micropropagation of Fraxinus mandshurica Rupr. Based on Liquid and Solid Alternate Culture

Fenghui Qi1,2, Wenxuan Wang1,2, Lin Liu1,2, Mingshuo Tang1,2, Feixiang Song1,2, Yaguang Zhan1,2()   

  1. 1.Key Laboratory of Saline-alkali Vegetation Ecology Restoration,Ministry of Education,Northeast Forestry University,Harbin 150040
    2.College of Life Science,Northeast Forestry University,Harbin 150040
  • Received:2021-03-08 Online:2022-03-20 Published:2022-02-22
  • Contact: Yaguang Zhan E-mail:yaguangzhan@126.com
  • About author:Qi Fenghui(1976—),female,PhD,senior engineer,master student supervisor, major in forest plant genetic improvement and cell engineering.
  • Supported by:
    This study was co-funded by Heilongjiang Provincial R&D Project on Provincial Applied Technology(GA19B201);Fundamental Research Funds for the Central Universities(2572020DR09)


A rapid and efficient regeneration system of liquid-solid alternate culture was developed for the tissue culture seedlings of Fraxinus mandshurica with lignificated stalk, aged leaves and dormant apical buds, and the axillary buds were germinated in a short time by liquid suspension culture, and the axillary buds were regenerated in vitro in solid culture to obtain the new tissue culture seedlings. On liquid and solid media supplemented of WPM with different plant growth regulators, axillary buds of F. mandshurica were induced to germinate and grow into seedlings. It was found that the axillary bud germination rate of F. mandshurica could reach 100% within 7-15 d after dark culture in WPM liquid medium supplemented with 0.6 mg·L-1 TDZ. After cutting the sprouted buds and inoculating them into WPM solid medium of 0.05 mg·L-1 TDZ and 0.6 mg·L-1 BA, axillary buds could grow into shoots after 1-2 subcultures with an average height of 2.64 cm and a multiplication coefficient of 4.04. After transplanting, the survival rate of rooting seedlings was 90% after 50 d. The establishment of the method could be conducive to the large-scale propagation of F. mandshurica, and the liquid-solid alternate circulation culture, simple, controllable, easy to operate, suitable for different production conditions and reduces the cost.

Key words: Fraxinus mandshurica Rupr., dormant bud, tissue culture, rapid reproduction

CLC Number: