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Bulletin of Botanical Research ›› 2022, Vol. 42 ›› Issue (1): 104-111.doi: 10.7525/j.issn.1673-5102.2022.01.011

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Tissue-specific Expression and Analysis of Exogenous Hormone Response of BpPIN5 Gene Promoter in Betula platyphylla

Yunli Yang, Chang Qu, Yang Wang, Guifeng Liu, Jing Jiang()   

  1. State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040
  • Received:2020-10-08 Online:2022-01-20 Published:2021-12-30
  • Contact: Jing Jiang E-mail:jiangjing1960@126.com
  • About author:Yang Yunli(1996—),female,postgraduate,mainly engaged in tree genetics and breeding research.
  • Supported by:
    The Innovation Project of State Key Laboratory of Tree(2020A01)


Being the polar carrier of IAA, PIN family proteins played a key role in plant embryonic development, organ development and tropic growth, especially in the formation of plant phyllodes, veins and vascular tissue differentiation.In order to identify the response characteristics BpPIN5 gene to exogenous hormones, the upstream 1447 bp sequence of BpPIN5 was cloned from the genome DNA of Betula platyphylla. The cis-acting elements in the sequence were predicted by PLACE online software. The results showed that the promoter sequence of BpPIN5 contained different types of auxin responsive elements such as auxin, gibberellin, Salicylic acid, abscisic acid, methyl jasmonate, ethylene respectively. Transgenic vector pro-BpPIN5::GUS to B. platyphylla was constructed. GUS histochemical staining analysis reflicted that BpPIN5 promoter had transcriptional activity in the tip of leaf crack, venule and root of B. platyphylla respectively. After transgenic Betula platyphylla was treated with IAA, GA, MeJA, SA and ABA respectively, the results showed that BpPIN5 promoter responded to the five hormones above at the crack edge of the first leaf, petiole and root tissue of the second leaf, and the response changes were basically the same. The results would help to clarify the function of BpPIN5 in B. platyphylla.

Key words: Betula platyphylla, auxin carrier, PINs, hormon response

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