Tissue-specific Expression and Analysis of Exogenous Hormone Response of BpPIN5 Gene Promoter in Betula platyphylla

doi:10.7525/j.issn.1673-5102.2022.01.011

Abstract

Abstract:

Being the polar carrier of IAA， PIN family proteins played a key role in plant embryonic development， organ development and tropic growth， especially in the formation of plant phyllodes， veins and vascular tissue differentiation.In order to identify the response characteristics BpPIN5 gene to exogenous hormones， the upstream 1447 bp sequence of BpPIN5 was cloned from the genome DNA of Betula platyphylla. The cis-acting elements in the sequence were predicted by PLACE online software. The results showed that the promoter sequence of BpPIN5 contained different types of auxin responsive elements such as auxin， gibberellin， Salicylic acid， abscisic acid， methyl jasmonate， ethylene respectively. Transgenic vector pro-BpPIN5：：GUS to B. platyphylla was constructed. GUS histochemical staining analysis reflicted that BpPIN5 promoter had transcriptional activity in the tip of leaf crack， venule and root of B. platyphylla respectively. After transgenic Betula platyphylla was treated with IAA， GA， MeJA， SA and ABA respectively， the results showed that BpPIN5 promoter responded to the five hormones above at the crack edge of the first leaf， petiole and root tissue of the second leaf， and the response changes were basically the same. The results would help to clarify the function of BpPIN5 in B. platyphylla.

Key words: Betula platyphylla, auxin carrier, PINs, hormon response

CLC Number:

S792.153

Yunli Yang, Chang Qu, Yang Wang, Guifeng Liu, Jing Jiang. Tissue-specific Expression and Analysis of Exogenous Hormone Response of BpPIN5 Gene Promoter in Betula platyphylla[J]. Bulletin of Botanical Research, 2022, 42(1): 104-111.

Figures/Tables 6

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顺式元件 CIS element	个数 Number	信号序列 Signal sequence	代码 Code	生物学功能 Biological function
ERELEE4	2	AWTTCAAA	S000037	乙烯响应元件
MYBGAHV	3	TAACAAA	S000181	参与赤霉素应答
GT1CONSENSUS	24	GRWAAW	S000198	细胞核调控蛋白GT-1结合位点，与光诱导基因，水杨酸诱导基因的表达有关
RAV1AAT	1	CAACA	S000314	病原菌及水杨酸响应的顺式调控元件
CATATGGMSAUR	4	CATATG	S000370	参与生长素反应
TGACGTVMAMY	1	TGACGT	S000377	顺式调控元件参与茉莉酸甲酯的反应
MYCCONSENSUSAT	25	CANNTG	S000407	ABA，低温应答，脱水相关MYB识别位点
MYB1AT	1	WAACCA	S000408	与脱水及ABA应答相关的MYB识别位点
GAREAT	3	TAACAAR	S000439	GARE（赤霉素响应元件）的结合位点
WRKY71OS	6	TGAC	S000447	病原菌及赤霉素应答相关的WRKY结合位点

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