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Bulletin of Botanical Research ›› 2023, Vol. 43 ›› Issue (3): 379-387.doi: 10.7525/j.issn.1673-5102.2023.03.007

• Physiology and Ecology • Previous Articles     Next Articles

Regulation of Salicylic Acid on Tolerance to Saline Alkali Stress at Seedling Stages of Betula platyphylla

Jingzhe WANG1, Chaokui NIU1, Xinyuan LIANG1, Chenjing SHEN1, Jing YIN1,2()   

  1. 2.College of Life Sciences,Northeast Forestry University,Harbin 150040
    1.Key Laboratory of Saline-alkali Vegetation Ecology Restoration,Ministry of Education,Northeast Forestry University,Harbin 150040
  • Received:2022-11-01 Online:2023-05-20 Published:2023-05-11
  • Contact: Jing YIN E-mail:yinjing20135@163.com
  • About author:WANG Jingzhe(2001—),female,undergraduate,mainly engaged in the research of plant salinity tolerance and secondary production regulation.
  • Supported by:
    Innovation and Entrepreneurship Training Program for College Students(202110225518);Basic Scientific Research Business Expenses of Central Universities(2572020DY22)


To investigate the effects of salicylic acid(SA) on growth and secondary production synthesis of Betula platyphylla seedling under saline alkali stress, three treatments were applied to birch seedlings: water treatment(control), 200 mmol·L-1 NaHCO3, 200 mmol·L-1 NaHCO3+360 μmol·L-1 SA. The relative conductivity, chlorophyll fluorescence parameters, osmotic content, anti-stress enzyme activity, the relative expression of key genes of triterpene synthesis pathway in the middle leaves of birch seedlings in response to different treatments were detected respectively, and the total triterpenoids, flavonoids and polyphenols of birch seedlings were analyzed. The results showed that: ①under the 200 mmol·L-1 NaHCO3 treatment, then 360 μmol·L-1 SA treatment significantly reduced the relative conductivity of the leaves of birch seedlings, improved the Fv/Fm value of chlorophyll fluorescence parameters of birch seedlings, increased the content of the osmotic regulator proline and soluble protein, and enhanced the activities of antioxidant enzymes(SOD, POD, CAT, APX) in birch seedlings to a certain extent. ②200 mmol·L-1 NaHCO3 saline alkali stress promoted the upregulation of the expression of key genes of triterpene synthesis pathway FPSSSSEBPX and BPW in birch seedlings, and significantly promoted the synthesis of polyphenols in birch seedlings in the early stage, and significantly promoted the accumulation of total triterpenoids and flavonoids in the late stage of saline-alkali stress(P<0.05). The application of SA under saline-alkali stress promoted the upregulation of HMGRFPSSE, and BPX gene expression in birch seedlings, and promoted the synthesis of flavonoids in the early stage of stress, and significantly promoted the accumulation of total triterpenes in the late stage of stress, which was 34% higher than that of the saline-alkali stress group and 47% higher than that of the water treatment on 7th day. Overall, under saline-alkali stress, SA with suitable concentration improves the tolerance to saline-alkali of birch seedlings by adjusting osmotic equilibrium, alleviating light inhibition, enhancing the antioxidant defense system of birch seedlings, and regulating substance metabolism.

Key words: Betula platyphylla, salicylic acid, tolerance to saline-alkali, secondary production

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