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Bulletin of Botanical Research ›› 2019, Vol. 39 ›› Issue (2): 287-293.doi: 10.7525/j.issn.1673-5102.2019.02.015

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Genetic Transformation and Resistance Analysis of BpBEE2 Gene from Betula platyphylla

YAN Bin, WU Dan-Yang, LI Hui-Yu   

  1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040
  • Received:2018-11-05 Online:2019-03-05 Published:2019-03-20
  • Supported by:
    National Key R&D Plan(2017YFD0600603)

Abstract: Brassinolide Enhanced Expression2(BEE2) genes belong to bHLH transcription factors family,and were involved in Brassinolide signal pathway.In this study, BpBEE2 gene was cloned using RT-PCR, and plant over-expression and inhibiting expression vector were constructed. Genetic transformation was operated through agrobacterium mediated, then mass growths of transgenic lines and non-transgenic line were analyzed under natural conditions and salt, drought stress. The length of 1 080 bp cDNA sequence was obtained, over-expression and inhibiting expression vectors were constructed successfully, and transgenic lines were obtained. Seedlings height of over-expression transgenetic line was higher than that of the control line, however, that of inhibiting expression lines was lower than that of the control line. BEE2 gene can ffect fresh weight of plants after salt and drought stress, and BpBEE2 is involved in the process of plant growth and development and plays a key role in drought and salt resistance response.

Key words: Betula platyphylla, BpBEE2 gene, vector construction, genetic transformation, drought and salt resistance

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