Welcome to Bulletin of Botanical Research! Today is Share:

Bulletin of Botanical Research ›› 2021, Vol. 41 ›› Issue (2): 251-261.doi: 10.7525/j.issn.1673-5102.2021.02.013

Previous Articles     Next Articles

Expression Profile Analysis ofXylem Development Regulated by BpNAC012 Gene from Betula platyphylla

Yi-Ping GUO, Jia-Xin LIU, Ying YU, Chao WANG, Chuan-Ping YANG()   

  1. State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040
  • Received:2020-06-17 Online:2021-03-20 Published:2021-01-05
  • Contact: Chuan-Ping YANG E-mail:yangcp@nefu.edu.cn
  • About author:GUO Yi-Ping(1994—),female,master,major in molecular mechanism of wood formation.
  • Supported by:
    Fundamental scientific research business fees of central universities(2572016DA01)


NAC transcription factors have been considered to be the switches that regulate the formation of plant secondary cell walls. Our previous work indicated that the gene BpNAC012 was involved in the secondary cell wall biosynthesis of birch. In order to identify the downstream target genes regulated by BpNAC012, transcriptome sequencing of stems from BpNAC012over-expression and RNAi plants was conducted, and the differentially expressed genes were identified compare to the wild type birch. The results showed that, in contrast to the wild type plants, 627 genes were up-regulated and 229 genes were down-regulated in the over-expression transgenic line, and 299 up-regulated genes and 207 down-regulated genes were identified in the RNAi lines. The expression changes of downstream genes in the overexpression transgenic lines were much greater than that in the RNAi plants. Inhibition of BpNAC012 expression significantly affected the expression of genes related to protein modification and transport. The differentially expressed genes were enriched in pathways such as receptor signaling pathways, nutritional metabolism, amino acid synthesis, and phenylpropane biosynthesis. In addition, BpNAC012 can regulate the expression of genes related to cellulose, lignin bio-synthesis and xylem development, as well as a variety of transcription factors. This study laid the foundation for further analysis of the molecular regulatory mechanism of BpNAC012 in secondary cell wall bio-synthesis of birch.

Key words: Betula platyphylla, BpNAC012, expression profile analysis, xylem development

CLC Number: