Genetic Transformation of BpERF98 Gene and Abiotic Stress Response of Transgenic Plant in Betula platyphylla

doi:10.7525/j.issn.1673-5102.2022.01.010

Abstract

Abstract:

Abiotic stresses seriously affected plant growth and development， and plants had evolved sophisticated internal molecular regulatory mechanisms to deal with， in which transcription factors played a vital role. In this study， BpERF98 gene was cloned from the leaves of Betula platyphylla and overexpressed using Agrobacterium-mediated. The physiological indicators of the transgenic lines and wild-type under low temperature， freezing damage and salt stress were measured respectively， the results showed that the malondialdehyde content and relative conductivity of the transgenic lines under abiotic stress were lower， while the SOD and POD enzyme activities were higher than those of the wild-type. The results showed that overexpression of BpERF98 gene could improve the tolerance of white birch to abiotic stress， and provided an important theoretical basis for studying the molecular mechanism under abiotic stress and molecular breeding for tolerance of white birch.

Key words: salt-tolerant, cryogenic, transgenic, transcription factor ERF

CLC Number:

S722.3+6

Qi Li, Siyu Yan, Su Chen. Genetic Transformation of BpERF98 Gene and Abiotic Stress Response of Transgenic Plant in Betula platyphylla[J]. Bulletin of Botanical Research, 2022, 42(1): 93-103.

Figures/Tables 10

Table 1

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引物 Primer	序列 Sequence
pROKⅡ-ERF98-F	ATCTCTAGAGCCACCATGGATGGAAAAAGAGGGGAAGGG
pROKⅡ-ERF98-R	ATCGGTACCTCAGTTCTTCTTTGTTTTGTAATC

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