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Bulletin of Botanical Research ›› 2018, Vol. 38 ›› Issue (4): 506-517.doi: 10.7525/j.issn.1673-5102.2018.04.004

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Growth Characteristics and Endogenous IAA Content of BpCUCt Transgenic Lines in Betula platyphylla

CHEN Chen, XING Bao-Yue, BIAN Xiu-Yan, XU Si-Jia, LIU Gui-Feng, JIANG Jing   

  1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040
  • Received:2018-02-24 Online:2018-07-15 Published:2018-07-21
  • Supported by:
    National Key R&D Plan(2017YFD0600603)

Abstract: To identify the function of BpCUCt in birch, we constructed two vectors that could be expressed in plants, namely ProCUC2::BpCUCt and 35S::BpCUCt. Meanwhile, we regarded zygotic embryo from elite birch trees as transgenic receptor, observed the changed phenotypes of transgenic lines and measured the endogenous IAA content. Compared to the wild type, the BpCUCt transgenic lines were with smaller height, lower growth characters and longer knot distances. However, the leaf margin of ProCUC2::BpCUCt lines were smoother than the wild type, transgenic lines of 35S::BpCUCt did not distinguish from the wild type. In view of the endogenous IAA content from the transgenic lines and wild type, the 83.33% of transgenic lines that was with more endogenous IAA content than wild type in the left leaf, the right leaf and the main vein. In conclusion, to some degree, the function of BpCUCt played similar roles in the function of BpCUC2, meanwhile, this gene was also related to endogenous IAA content in birch trees.

Key words: Betula platyphylla, BpCUCt, BpCUC2, endogenous IAA

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