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Bulletin of Botanical Research ›› 2019, Vol. 39 ›› Issue (1): 96-103.doi: 10.7525/j.issn.1673-5102.2019.01.012

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Cloning and Expression Analysis of BpSPL2 Promoter from Betula platyphylla

WANG Sheng-Yu, ZHANG Qi, ZHANG Zheng-Yi, HU Xiao-Qing, TIAN Jing, ZHANG Yong, LIU Xue-Mei   

  1. Life Science College, Northeast Forestry University, Harbin 150040
  • Received:2018-06-19 Online:2019-01-15 Published:2019-01-31
  • Supported by:
    Special Fund for Basic Scientific research operation Fee of Central University(2572015EA05)

Abstract: The SPL(SQUAMOSA promoter-binding protein-like) is a plant specific transcription factor, and the study showed that it plays an important role in the transformation of developmental stage, flower and fruit development. A 1 960 bp promoter sequence of BpSPL2 gene was cloned from Betula platyphylla genomic DNA using the method of PCR. The cis-regulatory elements were analyzed by PLACE and PlantCARE web tools. Multiple flowering elements, abiotic stress response elements and hormone-responsive elements were predicted in the promoter region. It indicated that it played an important role in plant growth and development and stress response.Further, the BpSPL2 promoter was inserted to pBI121 vector under control of the 35S promoter to generate the pBI121-BpSPL2 promoter::GUS recombinant construct, which was transient expressed in B.platyphylla and Arabidopsis thaliana seedlings by Agrobacterium tumefaciens mediated method, then investigated the expression pattern via histechemical GUS staining. BpSPL2 promoter could drive the expression of GUS gene in B.platyphylla and A.thaliana, and expressed in high level in leaves, buds, and roots of B.platyphylla while in high level in anthers, pistils, and leaves of A.thaliana. This study will provide a reference for further studies on the expression regulation and functional analysis of BpSPL2 gene in B.platyphylla.

Key words: Betula platyphylla, BpSPL2 promoter, cis-elements analysis, clone, expression analysis

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