Tissue Culture and Rapid Propagation of Trollius ledebouri

doi:10.7525/j.issn.1673-5102.2018.04.018

Abstract

Abstract: The seeds of Trollius ledebouri Reichb. were used by different concentration and treating time of GA₃ to promote germination, and their buds were inoculated on different types of medium with different hormone. A rapid propagation system by tissue culture was established and become basis for large-scale production of T.ledebouri. The results showed that:(1)the seeds of T.ledebouri Reichb was efficiently sterilized by 0.1% HgCl₂ for 20 min after soaking in 600 mg·L^-1 GA₃ solution for 48 h and the germination was high; (2)the suitable medium for multiplication of T.ledebouri was MS+0.5 mg·L^-1 6-BA+0.1 mg·L^-1 NAA; (3)the suitable rooting medium was MS without any hormone.

Key words: Trollius ledebouri Reichb., tisssue culture, rapid propagation

CLC Number:

Q949.95

ZHOU Fang, XU Jian-Qiu, AN Juan-Yan, JIANG Hong-Mao, WANG Zhen-Xi, ZHANG Tian-Xing, LI Kai-Long. Tissue Culture and Rapid Propagation of Trollius ledebouri[J]. Bulletin of Botanical Research, 2018, 38(4): 619-624.

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