Abstract: Using the apical buds of Gunnera manlcata as explants to induce the aseptic seedlings and then using the new leaves of aseptic seedling to induce the adventitious buds, in vitro culture and regeneration process of apical buds of G.manlcata were studied in this paper, and the quick propagation system in low cost was successfully established. The result showed: (1)Multiplication stage: MS+BA 1.0 mg·L^-1+NAA 0.1 mg·L^-1, cultured for 30 d, the stable multiplication rate was 3.80; (2)Induction stage: MS+ZT 1.0 mg·L^-1+NAA 0.1 mg·L^-1, which was proved to be superior to the others to be used to induce apical buds directly from the leaf explants, the induction rate reached 95%; (3)Rooting stage: 1/2MS+NAA 1 mg·L^-1+sugar 20 g·L^-1, the rooting rate reached 95%.

Key words: Gunnera manlcata L., tissue culture in vitro, rapid propagation