Abstract: We used the leaves of Carpinus betulus as explants to culture on WPM medium, and studied the effects of the different types and concentration of plant hormones on the induction, proliferation and differentition of callus. The rate of callus was 96.76%, highest at 0.5 mg·L^-1 2,4-D. The inducing rate of callus was 97.22% at 0.5 mg·L^-1 IBA. The inducing rate of callus was 95.03% at 0.5 mg·L^-1 NAA. The callus rate was 92.83% of WPM+0.5 mg·L^-1 BA+0.1 mg·L^-1 NAA+0.5 mg·L^-1 IBA and the callus rate was 96.44% of WPM+0.5 mg·L^-1 BA+0.5 mg·L^-1 NAA+0.01 mg·L^-1 IBA. The quality of calli preceded the calli indeced by single hormone, and two treatments were most suitable for inducing of leaves of European hornbeam. WPM+BA 2.0 mg·L^-1+KT 2.0 mg·L^-1+NAA 0.05 mg·L^-1 possessed the best proliferation effect and was appropriate for callus proliferation. WPM+BA 0.5 mg·L^-1+NAA0.1 mg·L^-1+2,4-D 0.1 mg·L^-1 had the best differentiation rate with the differentiation rate of 13.54%.

Key words: Carpinus belulus, leaves, callus, induction, differentiation