The Expression of VATPase Subunits in Overexpression ThVHAc1 Arabidopsis under Salt Stress

doi:10.7525/j.issn.1673-5102.2015.05.009

Abstract

Abstract: V-ATPase is a muti-subunits protein complex, the c subunit is mainly responsible for V-ATPase assembly and proton channel formation. We measured the expression profiles of V-ATPase subunits in exogenous overexpression ThVHAc1 Arabidopsis under salt by using real time RT-PCR. Twenty-eight V-ATPase subunits were all effected by exogenous ThVHAc1, and five Arabidopsis c subunits were down-regulated expression, indicating that ThVHAc1 affected the V-ATPase activity by altered the expression of V-ATPase all subunits. Between the expression of V-ATPase subunit and V-ATPase activity showed non-monolithic relationship, they were functioned together coordinately on V-ATPase to response to slat stress.

Key words: Tamarix hispida, expression analysis, c subunit, V-ATPase, ThVHAc1

CLC Number:

YANG Gui-Yan1,2；GUO Yu-Cong1；ZHAO Yu-Lin1；ZHANG Feng-Jiao1；GAO Cai-Qiu1*. The Expression of VATPase Subunits in Overexpression ThVHAc1 Arabidopsis under Salt Stress[J]. Bulletin of Botanical Research, 2015, 35(5): 692-695.

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