Abstract: We studied the tissue culture of Muscari botryoides Mill from four species of leaves and flower organs of Muscari aucheri ‘Dark Eyes’ with different concentrations of hormones, and established an efficient tissue culture system. With the variety of Muscari armeniacum, the most suitable sterilization time of mercuric chloride was 7 min. Callus could be induced from the leaves of every variety, and the appropriate medium was MS+2,4-D 0.5 mg·L^-1+TDZ 0.1 mg·L^-1. Different parts of one leaf showed high to low induction rate in the order of leaf base, leaf middle and leaf tip. Flower organs of M.aucheri ‘Dark Eyes’ showed high to low induction rate in the order of pedicels, petal and anthers. The optimal induction medium of anther was MS+2,4-D 0.1 mg·L^-1+6-BA 0.5 mg·L^-1.

Key words: Muscari botryoides Mill, leaf, flower organs, tissue culture