Salt Stress-related RcNAC22 Gene Cloning and Function Analysis in Rhodiolacrenulata

doi:10.7525/j.issn.1673-5102.2026.02.005

Abstract

Abstract:

This study cloned the RcNAC22 gene of R. crenulata and conducted bioinformatics analysis and functional verification. The results showed that the full length of RcNAC22 gene was 1 049 bp， containing two introns and three exons， and encoding 293 amino acids. Its protein relative molecular weight was 33 625.02， belonging to a hydrophilic protein， with relatively stable properties， no transmembrane domain or signal peptide， and subcellular localization in the nucleus， containing 32 phosphorylation sites. RcNAC22 contains one NAM domain （no apical meristem）， and the fourth domain is the transcriptional repression domain NARD region， and it was most closely related to that of R.kirilowii. RcNAC22 gene was expressed in all six tested organs （root， mature stem， young stem， leaf， apical bud， flower） but mainly in green organs （stem， leaf， apical bud）， and its expression could be induced by various abiotic stresses and plant hormones ABA and SA. RcNAC22 gene had no cytotoxicity to yeast cells， and both the full-length gene and truncated C-terminal had self-activation activity. In addition， using Salvia miltiorrhiza as the transgenic receptor to study the RcNAC22 gene effect on plant growth， the results showed that overexpression of RcNAC22 gene did not affect the growth and development of Salviamiltiorrhiza， but reduced the tolerance to salt stress. This study revealed the biological function of the RcNAC gene， providing a theoretical basis for further molecular biological research on R. crenulata.

Key words: Rhodiolacrenulata, transcription factor, gene cloning, salt stress

CLC Number:

S714.7

Lipeng ZHANG, Mei WU, Hongpeng WANG, Tianyu LI. Salt Stress-related RcNAC22 Gene Cloning and Function Analysis in Rhodiolacrenulata[J]. Bulletin of Botanical Research, 2026, 46(2): 246-258.

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引物名称 Primer name	正向引物（5′→3′） Forward primer （5′→3′）	反向引物（5′→3′） Reverse primer （5′→3′）
RcNAC22基因全长扩增	ATGTCGGCTACGAAGTCC	TCAGAGATTAAGCACGTTGAC
植物表达载体构建	cctaggATGTCGGCTACGAAGTCC	ggtcaccTCAGAGATTAAGCACGTTGAC
酵母表达载体构建	gaattcATGTCGGCTACGAAGTCC	ggatccTCAGAGATTAAGCACGTTGAC
基因N端序列酵母载体构建	gaattcATGTCGGCTACGAAGTCC	ggatccTCGCCGCTCTCTGCTCCAAC
基因C端序列酵母载体构建	gaattcATGTGGATCACGACGACGAAG	ggatccTCAGAGATTAAGCACGTTGAC
大花红景天内参基因	TACCCTTCATAGCACCCTCAGA	GAATGGCTTTCCGTGTCCC
丹参内参基因	GGTGCCCTGAGGTCCTGTT	AGGAACCACCGATCCAGACA