Table 1

PCR primers sequencesrelated to AFWRKY42 gene

Fig.1

PCR cloning and enzyme digestion of the AfWR-KY42 geneA.Electrophoresis of RT-PCR product（P）；B.#1，electrophoresis of identification of plasmid DNA double enzyme（BamHⅠ/HindⅢ） digestion of pMD18-T-AfWRKY42；M.DNA Marker

Fig.2

Bioinformation of AfWRKY42 proteinA.Structural mapping of AfWRKY42；B.Predicted secondary structure of AfWRKY42 proteinalpha helix is blue，beta corner is green，extended chain is red，and irregular curl is purple；C.AfWRKY42 phylogenetic tree，Cajanus cajan（CcWRKY47，CcWRKY42），Mucuna pruriens（MpWRKY42），Spatholobus suberectus（SsWRKY42），Melanaphis sacchari（GsWRKY42），Glycine max（GmWRKY42），Phaseolus vulgaris（PvPHAVU），Vigna unguiculata（VuWRKY31），Vigna angularis（VaWRKY6），Vigna radiata（VrWRKY6），Abrus precatorius（ApWRKY42），Cicer arietinum（CaWRKY42）；D.（a）Motif position map，（b）Motif shared motif map

Fig.3

Pattern of AfWRKY42 gene expressed in root，stem，epidermal，leaf，and inflorescence respectively

Fig.4

Expression characteristics of AfWRKY42 gene under saline stressA.Relative expression pattern of AfWRKY42 gene in Sophora japonica leaves under 150 mmol·L-1 NaCl stress treatment；B.Relative expression of AfWRKY42 in leaves；C.Relative expression pattern of AfWRKY42 gene in roots of Sophora japonica under 30 mmol·L-1 NaHCO3 stress treatment； D.Relative expression patterns of leaves* and ** indicate significant differences in relative expression between different stress times and the control at the P<0.05 and P<0.01 levels，respectively；Data are mean±standard error，the same as below

Fig.5

Subcellular localization of AfWRKY42 protein in Arabidopsis protoplastsA.Green fluorescent protein；B.Bright field；C.Merge of 35S：：GFP；D.Green fluorescent protein；Ｅ.Bright field；F.Merge of 35S：：GFP Of 35S：：AfWRKY42：：GFP

Fig.6

Identification of transgenic tobacco with AfWRKY42 geneA.PCR identification of AfWRKY42 transgenic tobacco（M.Marker；CK+.Positive control；CK－.Negative control；1-4.T0 generations of different transgenic lines）；B.AfWRKY42 transgenic tobacco relative expression analysis（WT.Wild-type plants；#1~#5.Transgenic plants）；The relative expressions are at the **P<0.01 level；The standard error of three biological replicates；* and ** indicate significant differences between transgenic strains and WT at the P<0.05 and P<0.01 levels，respectively，as follows；the same as below

Fig.7

Effect of salt （NaCl） stress on transgenic tobacco with AfWRKY42 geneA.Plant phenotype；B.Chlorophyll fluorescence；C.Chlorophyll content；D.Maximum light quantum efficiency（Fv/Fm）

Fig.8

Tolerance of transgenic tobacco with AfWRKY42 to NaHCO3 stressA.Plant phenotype；B.Chlorophyll fluorescence；C.Chlorophyll content；D.Maximum light quantum efficiency（Fv/Fm）