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Bulletin of Botanical Research ›› 2021, Vol. 41 ›› Issue (4): 564-572.doi: 10.7525/j.issn.1673-5102.2021.04.012

• Research report • Previous Articles    

Determination of Seed Vigor and Genetic Analysis of Foreign Genes in Different Transgenic Birch Hybrids

Dong-Lin LÜ1, Teng LI2, Yi-Wen GUO1, Jing JIANG1, Hai-Jiao HUANG1()   

  1. 1.State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040
    2.Liaoning Forestry Vocation-Technical College,Shenyang 110101
  • Received:2020-03-05 Online:2021-07-20 Published:2021-03-24
  • Contact: Hai-Jiao HUANG E-mail:haijiao_sea@163.com
  • About author:LÜ Dong-Lin(1996—),male,undergraduate degree,tree genetics and breeding.
  • Supported by:
    National Natural Science Foundation of China(31800556)

Abstract:

To explore the transmission mode of target gene in hybrid offspring of transgenic birch for further follow-up investigation, it is helpful to realize polymerization breeding for study on transgenic birch. In this study, the growth traits including thousand-seed weight,germination rate,germination potential and vigor index for offspring of transgenic birch were measured respectively. The offspring included families of T1 cross generation of 1-year-old BpAP1 and TabZIP transgenic birch, 5-year-old BpGH3.5 and BpCCR transgenic birch and half-sibling families of open pollination. The results showed that thousand-seed weight, seed vigor index and seedling height were all significantly different at 0.01 level among those families respectively, and family heritability of each trait was higher than 85.00%. Results showed that the transmission mode of target gene in T2 generation population does not accord with mendelian inheritance. The transfer efficiency of target genes of female and male gametes was about 30.00% and 50.00% respectively, however T2 hybrid polymerized with the 3 target genes was unattained. The random insertion of target gene reduced the seed vigor of T2 generation hybrid. We did not obtain birch hybrids with the 3 target genes. The polymerization of the target gene had an adverse effect on the growth and development of the hybrid offspring, and even death.

Key words: transgenic birch, seed vigor, pyramiding breeding, detection of target gene, inherited rules

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