Cloning and Functional Analysis of EgrWAT1 Gene in Eucalyptus grandis

doi:10.7525/j.issn.1673-5102.2023.04.013

Abstract

Abstract:

In order to explore the role of WALLS ARE THIN（WAT1） in wood formation and response to stress in woody plants， bioinformatics toolswas used for analysis， and quantitative Real-time PCR（qRT-PCR） was used to investigate the expression patterns of EgrWAT1L and EgrWAT1S in different tissues， internodes and in response to different stresses， and the gene EgrWAT1S and its other transcript EgrWAT1L were cloned from Eucalyptus grandis. The results showed that the EgrWAT1S was highly expressed in phloem， while EgrWAT1L was mainly expressed in roots， and the expression patterns were significantly different under methyl jasmonate（MeJA） and salicylic acid（SA） treatment， salt stress， phosphorus（P） and boron（B） deficiency， and even opposite under MeJA and SA. These results suggested that EgrWAT1L might affect EgrWAT1S expression through transcriptional regulation and further protein translation in response to hormone and stress treatments. The studies provided a basis for further elucidate the function in the growth and development of EgrWAT1 gene and also provided a possibility for future molecular breeding of Eucalyptus.

Key words: Eucalyptus grandis, WAT1, splice variant, gene cloning, expression pattern

CLC Number:

S792.39

Haonan ZHANG, Shanshan CHEN, Jianmin XU, Ping LUO, Xiaoping WANG, Zhiru XU, Chunjie FAN. Cloning and Functional Analysis of EgrWAT1 Gene in Eucalyptus grandis[J]. Bulletin of Botanical Research, 2023, 43(4): 601-611.

Figures/Tables 7

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引物名称 Primer name	引物序列（5′→3′） Primer sequences（5′→3′）
EgrWAT1 PCR F	AGGGCTTGCTCTGTGTTCCTG
EgrWAT1 PCR R	CATCCTGAGGATGATCTACGGTC
EgrWAT1S qRT-PCR F	CTCGGGATGGAGAAACTCAG
EgrWAT1S qRT-PCR R	CTTTTCCATGCGTCAAGACA
EgrWAT1L qRT-PCR F	TTCCCGATACTGTACTAGATCACC
EgrWAT1L qRT-PCR R	TCCATGCGTCAAGACATCAG
EgrEF2-F	TCCAATCCGAGTCGCTGTCATTGT
EgrEF2-R	TGATGAGCCTCTCTGGTTTGACCT
EgrWAT1 pROKII F	GCTCTAGAATGGAAAACGGCGCTTC
EgrWAT1 pROKII R	GGGGTACCCTACTCGACAGTAGTCACTG

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