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Bulletin of Botanical Research ›› 2022, Vol. 42 ›› Issue (6): 986-996.doi: 10.7525/j.issn.1673-5102.2022.06.008

• Molecular biology • Previous Articles     Next Articles

Cloning and Functional Analysis of miR398a from Chrysanthemum× grandiflora in Response to Salt Stress

Liran YUE1, Yingjie LIU1, Chenxu LIU2, Yunwei ZHOU3()   

  1. 1.College of Landscape Architecture,Northeast Forest University,Harbin 150040
    2.Dezhou Vocational and Technical College,Dezhou 253000
    3.College of Horticulture,Jilin Agricultural University,Jilin 130118
  • Received:2021-10-20 Online:2022-11-20 Published:2022-11-22
  • Contact: Yunwei ZHOU E-mail:dlzhyw@126.com
  • About author:YUE Liran(1978—),female,Ph.D,associate professor,engaged in Garden plant resources and Application.
  • Supported by:
    National Natural Science Foundation(31870687)


miRNAs played an important role in abiotic stress, and miR398a and pre-miR398a sequences were obtained from Small RNA database of Chrysanthemum×grandiflora. The sequence alignment showed that the cgr-miR398a was highly conserved with the identified miR398a sequences of other plants. Target genes prediction of cgr-miR398a showed that their annotation of targets include superoxide dismutase[Cu-Zn](CSD2), copper chaperone(CCS), A20/AN1-like zinc finger family protein(SAP8). The qPCR assay showed that the expression levels of miR398a was significantly decreased while target genes showed contrary trend during salt stress in Chrysanthemum×grandiflora. To explore the function of cgr-mir398a in response to salt stress, and an overexpression vector of cgr-mir398a was constructed and transformedinto Arabidopsis thaliana.The result showed that overexpression of the cgr-MIR398a in Arabidopsis could decrease seed germination under salt stress and salt tolerance at the adult seedling stage, therefore cgr-miR398a played a negative regulatory role in Arabidopsis in response to salt stress. The results provided a valuable reference for further exploring the functional mechanism of miR398a during salt stress in Chrysanthemum×grandiflora.

Key words: Chrysanthemum×grandiflora, cgr-miR398a, target gene, qPCR, salt stress

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