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Bulletin of Botanical Research ›› 2022, Vol. 42 ›› Issue (1): 112-120.doi: 10.7525/j.issn.1673-5102.2022.01.012

• Research Report • Previous Articles     Next Articles

Cloning and Expression Analysis of Transcription Factor LobHLH34 from Larix olgensis

Yuning Yang, Hao Dong, Shiwei Dong, Nairui Wang, Yue Song, Hanguo Zhang, Shujuan Li()   

  1. State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040
  • Received:2020-12-22 Online:2022-01-20 Published:2021-12-30
  • Contact: Shujuan Li E-mail:lishujuan@126.com
  • About author:Yang Yuning(1996—),female,postgraduate,Mainly engaged in larch genetic breeding research.
  • Supported by:
    Natural Science Foundation of Heilongjiang Province(C2017008);National Science and Technology Major Project(2018ZX 08020003-001-001)


In order to understand the function of transcription factor LobHLH34 and explore its expression pattern in different tissues and under different stress conditions, the full-length sequence of LobHLH34 gene was obtained from transcriptome data of root, stem and leaf of Larix olgensis, by using the primers designed. The complete open reading frame(ORF) of LobHLH34 gene was 696 bp and encoded 231 amino acids. Subcellular expression vector was constructed and transformed into protoplasts of Populus trichocarpa. The result showed that the LobHLH34 gene was located in the cell nucleus under the laser confocal microscope. The results of phylogenetic tree analysis showed that larch had the closest genetic relationship with Picea asperata and Selaginella tamariscina. By qRT-PCR, the tissue-specific expression of LobHLH34 gene and its response to abiotic stress were analyzed respectively. The results showed that LobHLH34 gene was expressed in the roots, stems and leave, and the lowest expression level was found in the stem and the highest relative expression in the leaf respectively. The expression level of LobHLH34 was different in different organs under NaCl, PEG and ABA stress. It was speculated that LobHLH34 gene could be involved in the process of plant growth, development and respond to stress, and it had special expressional pattern in different organs.

Key words: Larix olgensis, LobHLH34, gene cloning, bioinformatics analysis, expression analysis

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