Bulletin of Botanical Research ›› 2021, Vol. 41 ›› Issue (4): 626-632.doi: 10.7525/j.issn.1673-5102.2021.04.018
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Yuan GAO1, Wen-Xin XING2, Hui-Mei WANG1()
Received:
2020-04-10
Online:
2021-07-20
Published:
2021-03-24
Contact:
Hui-Mei WANG
E-mail:whm0709@163.com
About author:
GAO Yuan(1995—),female,master,mainly engaged in physiology of medicinal plants.
Supported by:
CLC Number:
Yuan GAO, Wen-Xin XING, Hui-Mei WANG. Macroporous Resin Purifying Polyphenols from Empetrum nigrum Aerial Parts[J]. Bulletin of Botanical Research, 2021, 41(4): 626-632.
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URL: https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2021.04.018
Table 1
Physical parameters of six types of macroporous resins
类型 Trade name | 极性 Polarity | 比表面积 Surface area (m2g) | 平均孔径 Average pore diameter(nm) | 粒径范围Particle diameter (mm) |
---|---|---|---|---|
HPD-100 | 非极性Nonpolarity | 650~700 | 9~10 | 0.3~1.25 |
X-5 | 非极性Nonpolarity | 500~600 | 29~30 | 0.25~0.84 |
AB-8 | 弱极性Weak polarity | 480~520 | 13~14 | 0.3~1.25 |
D101 | 弱极性Weak polarity | 500~550 | 9~10 | 0.25~0.84 |
HPD-600 | 极性Polarity | 550~600 | 8 | 0.30~1.20 |
NKA-Ⅱ | 极性Polarity | 160~200 | 14.5~15.5 | 0.30~1.25 |
Table 3
Moisture content, adsorption rate and desorption rate of six types of macroporous resin
类型 Trade name | 含水率 Moisture content (%) | 吸附率 Adsorption rate (%) | 解吸率 Desorption rate (%) |
---|---|---|---|
HPD-100 | 61.16±0.77 | 55.95±0.46 | 74.56±0.82 |
X-5 | 71.94±0.84 | 56.98±0.66 | 53.81±0.54 |
AB-8 | 73.27±0.28 | 62.93±0.84 | 23.75±0.98 |
D101 | 73.06±0.34 | 64.11±0.72 | 29.60±0.62 |
HPD-600 | 76.74±0.56 | 72.49±0.55 | 77.25±0.47 |
NKA-Ⅱ | 59.40±0.58 | 58.84±1.12 | 25.40±0.71 |
Table 4
The experiment design and results of Box-Behn-ken
序号 No. | A:上样浓度 Sample concentration (mg·mL-1) | B:乙醇浓度 Ethanol concentration (%) | C:洗脱流速 Elution flow rate (mL·min-1) | D:洗脱体积 Elution volume (BV) | 提取率 Extraction yield (mg·g-1) |
---|---|---|---|---|---|
1 | 0.80 | 70.00 | 0.50 | 1.00 | 68.85 |
2 | 0.60 | 70.00 | 1.00 | 1.00 | 72.92 |
3 | 0.80 | 60.00 | 0.50 | 2.00 | 168.24 |
4 | 0.80 | 80.00 | 1.00 | 3.00 | 180.26 |
5 | 0.80 | 60.00 | 1.00 | 3.00 | 211.00 |
6 | 0.80 | 80.00 | 0.50 | 2.00 | 175.51 |
7 | 0.80 | 70.00 | 1.00 | 2.00 | 212.12 |
8 | 0.80 | 60.00 | 1.00 | 1.00 | 77.67 |
9 | 0.80 | 70.00 | 1.00 | 2.00 | 200.94 |
10 | 0.60 | 80.00 | 1.00 | 2.00 | 87.01 |
11 | 0.80 | 70.00 | 0.50 | 3.00 | 224.69 |
12 | 0.80 | 70.00 | 1.00 | 2.00 | 195.63 |
13 | 1.00 | 80.00 | 1.00 | 2.00 | 165.72 |
14 | 1.00 | 70.00 | 1.00 | 1.00 | 94.56 |
15 | 0.80 | 60.00 | 1.50 | 2.00 | 129.67 |
16 | 0.80 | 80.00 | 1.50 | 2.00 | 186.40 |
17 | 1.00 | 70.00 | 1.50 | 2.00 | 199.26 |
18 | 0.80 | 80.00 | 1.00 | 1.00 | 61.02 |
19 | 0.80 | 70.00 | 1.00 | 2.00 | 216.31 |
20 | 0.60 | 60.00 | 1.00 | 2.00 | 101.05 |
21 | 1.00 | 60.00 | 1.00 | 2.00 | 174.95 |
22 | 1.00 | 70.00 | 0.50 | 2.00 | 211.28 |
23 | 0.60 | 70.00 | 1.00 | 3.00 | 155.66 |
24 | 1.00 | 70.00 | 1.00 | 3.00 | 216.31 |
25 | 0.60 | 70.00 | 0.50 | 2.00 | 125.30 |
26 | 0.80 | 70.00 | 1.50 | 3.00 | 175.51 |
27 | 0.80 | 70.00 | 1.00 | 2.00 | 224.13 |
28 | 0.60 | 70.00 | 1.50 | 2.00 | 74.37 |
29 | 0.80 | 70.00 | 1.50 | 1.00 | 86.79 |
Table 5
Analysis of variance of quadratic regression model
来源 Source | 平方和 Sum of squares | 自由度 df | 均方 Mean square | F值 F-value | P值 P-value | 显著性 Significance |
---|---|---|---|---|---|---|
模型Model | 82 747.21 | 14 | 5 910.51 | 14.61 | <0.000 1 | ?? |
A | 16 559.24 | 1 | 16 559.24 | 40.92 | <0.000 1 | ?? |
B | 3.70 | 1 | 3.70 | 9.135E-003 | 0.925 2 | |
C | 1 237.69 | 1 | 1 237.69 | 3.06 | 0.102 2 | |
D | 41 022.55 | 1 | 41 022.55 | 101.38 | < 0.000 1 | ?? |
AB | 5.78 | 1 | 5.78 | 0.014 | 0.906 5 | |
AC | 378.50 | 1 | 378.50 | 0.94 | 0.349 9 | |
AD | 380.45 | 1 | 380.45 | 0.94 | 0.348 7 | |
BC | 611.57 | 1 | 611.57 | 1.51 | 0.239 2 | |
BD | 49.63 | 1 | 49.63 | 0.12 | 0.731 4 | |
CD | 1 126.27 | 1 | 1 126.27 | 2.78 | 0.117 4 | |
A2 | 9 258.80 | 1 | 9 258.80 | 22.88 | 0.000 3 | ?? |
B2 | 6 964.46 | 1 | 6 964.46 | 17.21 | 0.001 0 | ?? |
C2 | 2 427.66 | 1 | 2 427.66 | 6.00 | 0.028 1 | ? |
D2 | 12 801.41 | 1 | 12 801.41 | 31.64 | < 0.000 1 | ?? |
残差Residual | 5 665.00 | 14 | 404.64 | |||
失拟项Lack of fit | 5 132.61 | 10 | 513.26 | 3.86 | 0.102 6 | |
纯误差Pure error | 532.40 | 4 | 133.10 | |||
总变异Cor total | 88 412.21 | 28 | ||||
R2 | 0.935 9 | R2Adj | 0.871 9 |
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