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Bulletin of Botanical Research ›› 2020, Vol. 40 ›› Issue (5): 775-781.doi: 10.7525/j.issn.1673-5102.2020.05.017

• Scientific Notes • Previous Articles     Next Articles

Optimization of Ultrasound-assisted Extraction of Dihydromyricetin from Seeds of Hovenia dulcis Thunb. Using Response Surface Methodology

ZHANG Xiao-Nan1,2, ZHU Hong-Wei2, ZHAO Shan-Bo2, BAI Chen2, SHI Ya-Nan2, XU Hao-Peng2, YIN Lian1, HU Shu-Min1, YIN Hua1   

  1. 1. State key laboratory of beer biological fermentation engineering, Qingdao beer co., LTD., Qingdao 266071;
    2. Food Science College, Northeast Agricultural University, Harbin 150036
  • Received:2019-09-26 Online:2020-09-05 Published:2020-07-10
  • Supported by:
    Heilongjiang Natural Fund General Project(C2018025)

Abstract: Dihydromyricetin is an important component in the seeds of Hovenia dulcis Thunb. The extraction of dihydromyricetin from seeds of H.dulcis by ultrasound-assisted method was carried out on the basis of single factor investigation of ethanol volume fraction, ultrasound irradiation power, extraction temperature, liquid-solid ratio and ultrasound irradiation time. The Box-Behnken response surface design method was used to establish a quadratic polynomial model of ultrasound irradiation power, ultrasound irradiation time and liquid-solid ratio, and optimized extraction process. The optimum conditions for the extraction were as follows:ethanol volume fraction of 60%, ultrasonic irradiation power of 140 W, ultrasonic irradiation time of 30 min, liquid-solid ratio of 20.5 mL·g-1, and extraction temperature of 40℃. Under these optimal conditions, the yield of dihydromyricetin was 2.14±0.09 mg·g-1. The extraction method is simple, rapid, and high in efficiency, which is beneficial to the comprehensive processing and utilization of the H.dulcis resources.

Key words: Hovenia dulcis Thunb., seed, ultrasound-assisted extraction, dihydromyricetin, response surface methodology

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