Welcome to Bulletin of Botanical Research! Today is Share:

Bulletin of Botanical Research ›› 2021, Vol. 41 ›› Issue (4): 626-632.doi: 10.7525/j.issn.1673-5102.2021.04.018

• Research report • Previous Articles    

Macroporous Resin Purifying Polyphenols from Empetrum nigrum Aerial Parts

Yuan GAO1, Wen-Xin XING2, Hui-Mei WANG1()   

  1. 1.Key Laboratory of Forest Plant Ecology,College of Chemistry,Chemical Engineering and Resource Utilization,Northeast Forestry University,Harbin 150040
    2.College of Life Sciences,Northeast Forestry University,Harbin 150040
  • Received:2020-04-10 Online:2021-07-20 Published:2021-03-24
  • Contact: Hui-Mei WANG E-mail:whm0709@163.com
  • About author:GAO Yuan(1995—),female,master,mainly engaged in physiology of medicinal plants.
  • Supported by:
    Fundamental Research Funds for the Central Universities(2572017EA03)

Abstract:

In order to optimize the process of polyphenols by macroporous resin from Empetrum nigrum aerial parts, moisture content, adsorption rate and desorption rate of six resins(HPD-100, X-5, AB-8, D101, HPD-600 and NKA-Ⅱ) were investigated, and the resin with the best effect was selected. Four factors(sample concentration, ethanol concentration, elution flow rate and elution volume) that have a great influence on the purification process were selected, and the response surface method was used to analyze the optimal process. HPD-600 macroporous resin had the best purification effect on polyphenols yield. The optimal extraction conditions were as follows: sample concentration was 0.84 mg·mL-1, ethanol concentration was 62.15%, elution flow rate was 0.67 mL·min-1, and elution volume was 2.71 BV. Under these conditions, the yield of polyphenols was 229.18 mg·g-1, the purity of polyphenols was increased from 8.11% to 22.56%, and the recovery rate was 67.78%.

Key words: Empetrum nigrum, polyphenols, macroporous resin, response surface method, purification process

CLC Number: