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Bulletin of Botanical Research ›› 2021, Vol. 41 ›› Issue (4): 626-632.doi: 10.7525/j.issn.1673-5102.2021.04.018

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Macroporous Resin Purifying Polyphenols from Empetrum nigrum Aerial Parts

Yuan GAO1, Wen-Xin XING2, Hui-Mei WANG1()   

  1. 1.Key Laboratory of Forest Plant Ecology,College of Chemistry,Chemical Engineering and Resource Utilization,Northeast Forestry University,Harbin 150040
    2.College of Life Sciences,Northeast Forestry University,Harbin 150040
  • Received:2020-04-10 Online:2021-07-20 Published:2021-03-24
  • Contact: Hui-Mei WANG E-mail:whm0709@163.com
  • About author:GAO Yuan(1995—),female,master,mainly engaged in physiology of medicinal plants.
  • Supported by:
    Fundamental Research Funds for the Central Universities(2572017EA03)


In order to optimize the process of polyphenols by macroporous resin from Empetrum nigrum aerial parts, moisture content, adsorption rate and desorption rate of six resins(HPD-100, X-5, AB-8, D101, HPD-600 and NKA-Ⅱ) were investigated, and the resin with the best effect was selected. Four factors(sample concentration, ethanol concentration, elution flow rate and elution volume) that have a great influence on the purification process were selected, and the response surface method was used to analyze the optimal process. HPD-600 macroporous resin had the best purification effect on polyphenols yield. The optimal extraction conditions were as follows: sample concentration was 0.84 mg·mL-1, ethanol concentration was 62.15%, elution flow rate was 0.67 mL·min-1, and elution volume was 2.71 BV. Under these conditions, the yield of polyphenols was 229.18 mg·g-1, the purity of polyphenols was increased from 8.11% to 22.56%, and the recovery rate was 67.78%.

Key words: Empetrum nigrum, polyphenols, macroporous resin, response surface method, purification process

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