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Bulletin of Botanical Research ›› 2020, Vol. 40 ›› Issue (4): 635-640.doi: 10.7525/j.issn.1673-5102.2020.04.019

• Scientific Notes • Previous Articles    

Response Surface Optimized Enzymatic Hydrolysis-microwave Assisted Extraction Process of Mulberry Leaf Polysaccharide

ZHU Ya-Wei1,2, WANG Li-Tao3, AN Juan-Yan3, Lü Mu-Jie3, SUN Jian-Hang4, GUO Na1,2, FU Yu-Jie1,2   

  1. 1. Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040;
    2. College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040;
    3. College of Forestry, Beijing Forestry University, Beijing 100083;
    4. Department of Neurology, The First Hospital Affiliated of Harbin Medical University, Harbin 150000
  • Received:2020-03-06 Online:2020-07-05 Published:2020-06-12
  • Supported by:
    National Key R&D Program of China(2016YDF0600805);Applied Technology Research and Development Project of Heilongjiang Province(GX18B003);111 Innovation and Intelligence Introduction Project(B20088)

Abstract: The response surface analysis was used to optimize the process of extracting polysaccharide from mulberry leaves by enzymatic hydrolysis-microwave assisted method.Based on the single factor test, the Box-Behnken method was used to study the effects of liquid-solid ratio, extraction time and extraction temperature on the extraction rate of mulberry leaf polysaccharides. The fitting equation was significant, and the optimal extraction conditions of mulberry leaf polysaccharides were finally determined as follows:enzyme content 2%, enzymolysis pH6, enzymolysis temperature 50℃, enzymolysis time 20 min, liquid-solid ratio 15 mL·g-1, extraction time 13 min, and extraction temperature 76℃.Under these conditions, the actual extraction rate of mulberry leaf polysaccharide was 15.23%, which was closed to the theoretical simulation value of 15.12%. Therefore, the established model was true and reliable. The method can be used for extracting polysaccharide components in mulberry leaves with the advantages of simple process, low cost and high application value.

Key words: Morus alba L., mulberry leaves polysaccharides, enzymatic hydrolysis-microwave assisted extraction, response surface methodology, extraction process

CLC Number: