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Bulletin of Botanical Research ›› 2019, Vol. 39 ›› Issue (4): 529-538.doi: 10.7525/j.issn.1673-5102.2019.04.007

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Cloning and Expression Analysis of FmWRKY44 Gene in Fraxinus mandshurica

LI Xin-Yu1, HE Li-Ming1, ZHAN Ya-Guang1,2   

  1. 1. College of Life Sciences, Northeast Forestry University, Harbin 150040;
    2. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040
  • Received:2018-12-05 Online:2019-07-05 Published:2019-07-03
  • Supported by:
    National Key Research and Development Program(2017YFD0600605-01-02)

Abstract: To clone the FmWRKY44 gene from Fraxinus mandshurica and explore its role in abiotic stress and hormone stress, specific primers were designed using the sequences of the variceal drought-transcriptome, the complete ORF sequence of FmWRKY44 gene was cloned, and bioinformatics analysis was carried out on the sequence and its coded products. The expression pattern of FmWRKY44 was analyzed using qRT-PCR. A WKRY gene of F. mandshurica was cloned, encoding 1 383 bp, encoding 460 amino acid. It was found to be a stable hydrophilic protein by the analysis of its encoded protein. The subcellular localization was mainly predicted in the nucleus, and the conserved domain and homology were analyzed. It belonged to the WRKY class I family and was named FmWRKY44. By qRT-PCR analysis, FmWRKY44 was highly expressed in the seeds. Different degrees of response to low temperature, high temperature, salt and drought four abiotic stresses. FmWRKY44 and NAA, ABA, GA3, JA phytohormone responses were also found. FmWRKY44 gene of F. mandshurica positively responds to low temperature and high temperature stress.

Key words: Fraxinus mandshurica, WRKY gene, gene cloning, expression analysis of gene

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