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Bulletin of Botanical Research ›› 2019, Vol. 39 ›› Issue (4): 521-528.doi: 10.7525/j.issn.1673-5102.2019.04.006

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Expression Pattern of PagC3H3 Gene in Populus alba×P.glandulosa

FAN Er-Qin, LIU Cai-Xia, FU Peng-Yue, YANG Chuan-Ping, QU Guan-Zheng   

  1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040
  • Received:2019-01-03 Online:2019-07-05 Published:2019-07-03
  • Supported by:
    National Natural Science Foundation of China(31670675);Ministry of Education New Century Excellent Talent Support Program(NCET-12-0808)

Abstract: As the main component of wood, lignin is usually polymerized from three monomers. During its biosynthesis, a total of ten enzyme families are involved in the conversion of phenylalanine to monomeric lignin, of which C3H is the key control enzymes in the hydroxylation process of p-coumaroyl CoA to caffeoyl CoA and in the formation of G/S monomer. Exploring the expression pattern of PagC3H3 gene is of great significance for further understanding of the function of this gene. In this study, the tissue-specific expression of PagC3H3 gene was analyzed by quantitative PCR; the promoter sequence of PagC3H3 with a length of 2 035 bp was cloned, and it was predicted to contain multiple cis-acting elements. At the same time, the promoter sequence of PagC3H3 was constructed to construct a plant expression vector pBI121-PagC3H3pro::GUS for transient transformation of Arabidopsis thaliana. The expression of PagC3H3 gene was higher in the roots, middle stem segments and basal stem segments of Populus alba×P. grandulos; transient transformation of A. thaliana showed that GUS activity was stronger in hypocotyls and roots, suggesting that PagC3H3 gene plays a role in lignin synthesis.

Key words: Populus alba×P. grandulos, PagC3H3 gene, promoter, Arabidopsis thaliana, transient transformation, GUS detection

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