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Bulletin of Botanical Research ›› 2019, Vol. 39 ›› Issue (1): 156-160.doi: 10.7525/j.issn.1673-5102.2019.01.019

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Sequencing and Assembly of Populus simonii×P.nigra Genome and SSR Analysis

ZHOU Yu-Min1, WANG Sui2, LIU Yi2, LI Kai-Long2, YOU Xiang-Ling2   

  1. 1. Hubei Ecology Polytechnic College, Wuhan 430200;
    2. Northeast Forestry University, Harbin 150040
  • Received:2018-03-04 Online:2019-01-15 Published:2019-01-31
  • Supported by:
    Supported by the National Natural Science Foundation of China(31670675)

Abstract: Populus simonii×P.nigra, which is the hybrid crossed by P.simonii and P.nigra, inherits the advantages of fast growth and strong resistance from their parents. In this study, the genome sequence of Populus simonii×P.nigra was preliminarily sequenced and assembled by the next generation sequencing(NGS) technology. The SSR sequences were identified and analyzed. We hope this study can provide a reference for classification and phenotypic correlation of Populus simonii×P.nigra. The results show that we total assembled 368.96 Mbp genome sequence, which contain 366 876 contigs. SSR analysis was performed on 21 788 non-redundant contigs which were not less than 2 000 bp. In total, 18 111 SSR sequences were identified, and most of them are one, two or three nucleosides acid repeat motif. The primers were designed for the obtained SSR sequences, and a total of 12 838 primers were obtained for future experiments.

Key words: Populus simonii×P.nigra, genome, SSR

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