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Bulletin of Botanical Research ›› 2022, Vol. 42 ›› Issue (5): 790-801.doi: 10.7525/j.issn.1673-5102.2022.05.010

• Molecular biology • Previous Articles     Next Articles

Identification of Glutathione S-transferase Gene Family in Sugar Beet and the Response to Cadmium Stress

Xin’ai ZHONG1,2, Shiqi MENG1,2, Wanting Zhou1,2, Qi YAO1,3, Qiong ZHANG1,2, Wang XING1,2, Dali LIU1,2()   

  1. 1.National Beet Medium-term Gene Bank,Heilongjiang University,Harbin 150080
    2.Key Laboratory of Sugar Beet Genetics and Breeding,College of Advanced Agriculture and Ecological Environment,Heilongjiang University,Harbin 150080
    3.Key Laboratory of Molecular Biology,School of Life Sciences,Heilongjiang University,Harbin 150080
  • Received:2022-02-13 Online:2022-09-20 Published:2022-09-15
  • Contact: Dali LIU E-mail:daliliu_hlju@163.com
  • About author:ZHONG Xin’ai(1998—),female,graduate student,mainly engaged in crop molecular biology research.
  • Supported by:
    This work were supported by the National Sugar Beet Germplasm Resources Platform(NCGRC-2021-017);Ministry of Agriculture and Rural Areas programme(19210157);Identification, Evaluation, Cataloging and Warehousing of Sugar Beet Germplasm Resources programme(19210911);Safe Preservation of Sugar Beet Germplasm Resources(19211031);National sugar industry technology system project(CARS-170102);Natural Science Foundation of Heilongjiang Province of China(LH2019C057);Scientific Research Foundation of the Higher Education Institutions of Heilongjiang Province(KJCX201920)


To clarify the potential function of Glutathione-S-transferase gene(GST) family in sugar beet(Beta vulgaris) growth and development and abiotic stress, the sugar beet BvGSTs was identified, and the physical and chemical properties, evolutionary relationship, homeopathic elements, chromosome location and transcriptional expression characteristics under cadmium stress were comprehensively analyzed, respectively. The results showed that there were 52 members of BvGSTs in sugar beet genome, which were distributed in seven subfamilies; The molecular weight of the members ranged from 17-28 kDa, and the theoretical isoelectric point(pI) was between 5.16-6.77, and most of the members were located in cytoplasm. There were nine motifs found in BvGSTs, among which motif eight was unique to Phi subfamily. Among all the members, 30 BvGSTs were distributed in eight chromosomes respectively, with four tandem repeats. According to cis-acting element analysis, BvGSTs might be involved in a variety of biotic and abiotic stress responses. Transcriptome analysis showed that all of 52 BvGSTs were related to response of sugar beet to cadmium stress. The expression of most members of Tau subfamily under ground parts was positively regulated by cadmium stress; Phi subfamily above ground parts was significantly induced by cadmium stress, and the expression of Tau family was inhibited. The result of qRT-PCR showed that the transcripts of the four differentially expressed BvGSTs were regulated by cadmium stress, which was consistent with the results of transcriptome sequencing. These results would play a foundamental role in further study on the biological function of sugar beet glutathione S-transferase under cadmium stress.

Key words: sugar beet, genome-wide identification, glutathione S-transferase(GST), cadmium stress

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