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Bulletin of Botanical Research ›› 2022, Vol. 42 ›› Issue (6): 964-975.doi: 10.7525/j.issn.1673-5102.2022.06.006

• Systematic and Evolutionary • Previous Articles     Next Articles

Comparison of Codon Characteristics and Phylogeny of Chloroplast Genome between Ranunculaceae and Paeoniaceae

Yingtong MU, Dongchang FAN, Lijuan LÜ, Xiajie LI, Jingshi LU, Xiaoming ZHANG()   

  1. College of Grassland,Resources and Environment,Inner Mongolia Agricultural University,Huhhot 010019
  • Received:2022-03-29 Online:2022-11-20 Published:2022-11-22
  • Contact: Xiaoming ZHANG E-mail:bagenna123@aliyun.com
  • About author:MU Yingtong(1997—),male,graduate,major in plant systematic and evolution.
  • Supported by:
    Natural Science Foundation of Inner Mongolia(2019MS03052)


To clarify the codon usage pattern and phylogenetic relationship of the chloroplast genome between Ranunculaceae and Paeoniaceae, 33 species of Ranunculaceae and seven species of Paeoniaceae were used as materials, and the analysis software CodonW, online software CUSP, and R software were used to analyze the codon characteristics of chloroplast genes, while the MAFFT software, MEGA software were used for phylogenetic analysis respectively. The results showed that the chloroplast genomes of Paeoniaceae and Ranunculaceae (except Columbine) had a high consistency of high frequency codons, with 29 high frequency codons, which were basically biased towards ending with A/U, but the optimal codon were different. The codon bias of the chloroplast genome of Ranunculaceae and Paeoniaceae was mainly influenced by natural selection, and the codon bias of the chloroplast genome of Paeoniaceae was more affected by natural selection than that of Ranunculaceae. The phylogenetic relationship based on the complete chloroplast genome sequence and the CDS sequence of the chloroplast genome showed that although the phylogenetic relationship based on the complete chloroplast genome sequence and the chloroplast genome CDS sequence of Paeoniaceae was different, they could be divided into the peony group and the peony group. The phylogenetic relationship of Ranunculaceae based on the entire chloroplast genome sequence did not conform to the classification relationship of Chinese flora, but it supported the division of Ranunculaceae into four subfamilies and 14 families. The analysis results supported that Paeoniaceae were independent from Ranunculaceae and Ranunculus, and were classified into Saxifraga.

Key words: Ranunculaceae, Paeoniaceae, chloroplast genome, codons, phylogenetic

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