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Bulletin of Botanical Research ›› 2023, Vol. 43 ›› Issue (1): 120-130.doi: 10.7525/j.issn.1673-5102.2023.01.013

• Molecular biology • Previous Articles     Next Articles

Complete Chloroplast Genome Structure and Characterization of Syringa villosa subsp. wolfii

Yongchang LU1,2, Xin ZHANG1,2, Luyan ZHANG1,3, Jiuli WANG1()   

  1. 1.Key Laboratory of Plant Resources of Qinghai-Tibet Planteauin Chemical Research,Xining 810007
    2.Qinghai Tibetan-medicine Creation Engineering Technology Research Center,Xining 810007
    3.School of Life Sciences,Liaoning University,Shenyang 110036
  • Received:2022-06-17 Online:2023-01-20 Published:2022-12-23
  • Contact: Jiuli WANG E-mail:wang_jiul@163.com
  • About author:LU Yongchang(1965—),male,master,professor,major in Tibetan medicine quality control.
  • Supported by:
    Scientific and technological Achievements Transformation Project of Qinghai Province(2020-NK-171);High-level Talent Program of Qinghai Minzu University(2021XJG17);Thousand High-end Innovative Talents Plan of Qinghai Province


To clarify the basic characteristics of the complete chloroplast genome of Syringa villosa subsp. wolfii, the sequence information of chloroplast genome was analyzed by high-throughput sequencing technology, and its systematic evolution position was discussed. The results showed that:(1)The total length of the S. villosa subsp. wolfii chloroplast genome was 156 517 bp, which had a typical tetrad structure and had 131 functional genes, including 36 tRNA genes, 8 rRNA genes and 87 protein-coding genes respectively. (2)The relative synonymous codon usage(RSCU) analysis of the protein-coding region of the chloroplast genome showed that there were 31 codons with RSCU>1, of which 21 ended with A/U base; and there were 34 codons with RSCU<1, including 22 codons ending with G/C base. (3)In the chloroplast genome of S. villosa subsp. wolfii, 334 scattered repeats were detected, including 170 forward repeats and 164 palindromic repeats, and 227 SSR loci were discovered, and PCR primers were designed successfully for 226 of these loci. (4)Phylogenetic tree analysis based on the maximum likelihood method showed that S. villosa subsp. wolfii was the most closely to the S. yunnanensis. In this study, repeated sequences, IR boundaries and phylogeny of chloroplast genome of S. villosa subsp. wolfii were analyzed to provide reference data for molecular markers, phylogenetic classification, species identification and evaluation and DNA barcode development.

Key words: Syringa villosa subsp. wolfii, chloroplast genome, RSCU, sequence repeats, phylogeny

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