Effects of Melatonin on Flavonoids Content and Related Gene Expression Levels of Gardenia under Dark Conditions

doi:10.7525/j.issn.1673-5102.2018.04.010

Abstract

Abstract: Gardenia leaves were used as materials to study the effect of melatonin on flavonoid content and related gene expression in Gardenia leaves under dark conditions by transcriptome sequencing and liquid chromatography. The melatonin not only delayed the senescence of Gardenia, but also delayed the yellowing of leaves by reducing the accumulation of flavonoids through inhibiting the expression of related genes such as flavonoids biosynthetic pathways. Our experiment provided practical guidance for retarding the yellowing of Gardenia leaves under dark stress, and the reference to the use of melatonin to control and delay the yellowing of leaves.

Key words: Gardenia, dark stress, melatonin, flavonoids, transcriptome, HPLC

CLC Number:

Q949.781.1

WANG Rong, CHENG Meng-Lin, LIU Hui-Na, ZHAO Da-Qiu, TAO Jun. Effects of Melatonin on Flavonoids Content and Related Gene Expression Levels of Gardenia under Dark Conditions[J]. Bulletin of Botanical Research, 2018, 38(4): 559-567.

References

1. 萧浪涛,王三根. 植物生理学[M]. 北京:中国农业出版社,2003.Xiao L T,Wang S G. Plant physiology[M]. Beijing:China Agricultural Press,2003.
2. Lee D W. Anthocyanins in autumn leaf senescence[J]. Advances in Botanical Research,2002,37:147-165.
3. 于晓南,张启翔. 彩叶植物多彩形成的研究进展[J]. 园艺学报,2000,27(S1):533-538.Yu X N,Zhang Q X. Review of researches on leaf color changing of color-leafed plants[J]. Acta Horticulturae Sinica,2000,27(S1):533-538.
4. Lev-Yadun S,Yamazaki K,Holopainen J K,et al. Spring versus autumn leaf colours:evidence for different selective agents and evolution in various species and floras[J]. Flora-Morphology,Distribution,Functional Ecology of Plants,2012,207(1):80-85.
5. 李云飞,李彦慧,王中华,等. 土壤干旱胁迫对紫叶矮樱叶片呈色的影响[J]. 生态学报,2009,29(7):3678-3684.Li Y F,Li Y H,Wang Z H,et al. Effect of soil drought stress on leaf coloration-emerging of Prunus cistenena cv. pissardii[J]. Acta Ecologica Sinica,2009,29(7):3678-3684.
6. 张平. 几种常见彩叶植物的色素组成与叶色关系的研究[J]. 山东林业科技,2008(3):14-16.Zhang P. Study on the relationship between pigment composition and leaf color of several common colorful plants[J]. Shandong Forestry Science and Technology,2008(3):14-16.
7. 孟祥春,彭建宗,王小菁. 光和糖对非洲菊花色素苷积累及CHS、DFR基因表达的影响[J]. 园艺学报,2007,34(1):227-230.Meng X C,Peng J Z,Wang X J. Anthocyanin accumulation and CHS,DFR gene expression regulated by light and sugar in Gerbera hybrida ray floret[J]. Acta Horticulturae Sinica,2007,34(1):227-230.
8. 王帅. 设施葡萄延迟栽培叶片衰老生理及抗衰老技术研究[D]. 北京:中国农业科学院,2015.Wang S. Study on leaf senescence physiology and anti-aging technology of grape cultured in protected and delayed cultivation[D]. Beijing:Chinese Academy of Agricultural Sciences,2015.
9. 谭瑶,王文举,张亚红. 设施葡萄延后栽培中赤霉素处理对叶片衰老的影响[J]. 中外葡萄与葡萄酒,2007,(4):18-20,23.Tan Y,Wang W J,Zhang Y H. Effects of gibberellic acid on the senescence of grapevine leaves in autumn-prolonging cultivation[J]. Sino-Overseas Grapevine & Wine,2007,(4):18-20,23.
10. 丁银宝,吴惠青. 栀子花的应用及栽培管理技术[J]. 安徽农学通报,2012,18(11):160-161.Ding Y B,Wu H Q. Application of Gardenia and cultivation and management techniques[J]. Anhui Agricultural Science Bulletin,2012,18(11):160-161.
11. 韩建萍,陈士林,张文生,等. 栀子遗传多样性及遗传分化的RAPD分析[J]. 中国药学杂志,2007,42(23):1774-1778.Han J P,Chen S L,Zhang W S,et al. Study on genetic diversity and differentiation of Gardenia jasminoides Ellis using RAPD Markers[J]. Chinese Pharmaceutical Journal,2007,42(23):1774-1778.
12. Reiter R J. Oxidative processes and antioxidative defense mechanisms in the aging brain[J]. The FASEB Journal,1995,9(7):526-533.
13. Stehle J H,Saade A,Rawashdeh O,et al. A survey of molecular details in the human pineal gland in the light of phylogeny,structure,function and chronobiological diseases[J]. Journal of Pineal Research,2001,51(1):17-43.
14. Balzer I,Hardeland R. Melatonin in algae and higher plants-possible new roles as a phytohormone and antioxidant[J]. Plant Biology,1996,109(3):180-183.
15. Bajwa V S,Shukla M R,Sherif S M,et al. Role of melatonin in alleviating cold stress in Arabidopsis thaliana[J]. Journal of Pineal Research,2014,56(3):238-245.
16. Liang C Z,Zheng G Y,Li W Z,et al. Melatonin delays leaf senescence and enhances salt stress tolerance in rice[J]. Journal of Pineal Research,2015,59(1):91-101.
17. Li C,Wang P,Wei Z W,et al. The mitigation effects of exogenous melatonin on salinity-induced stress in Malus hupehensis[J]. Journal of Pineal Research,2012,53(3):298-306.
18. Li M Q,Hasan M K,Li C X,et al. Melatonin mediates selenium-induced tolerance to cadmium stress in tomato plants[J]. Journal of Pineal Research,2016,61(3):291-302.
19. Audic S,Claverie J M. The significance of digital gene expression profiles[J]. Genome Research,1997,7(10):986-995.
20. 叶兴乾,徐贵华,方忠祥,等. 柑橘属类黄酮及其生理活性[J]. 中国食品学报,2008,8(5):1-7.Ye X Q,Xu G H,Fang Z X,et al. Citrus flavonoids and their physiological activity[J]. Journal of Chinese Institute of Food Science and Technology,2008,8(5):1-7.
21. Harborne J B,Baxter H. The handbook of natural flavonoids[M]. New York:John Wiley & Sons,1999.
22. 古勇,李安明. 类黄酮生物活性的研究进展[J]. 应用与环境生物学报,2006,12(2):283-286.Gu Y,Li A M. Progress in research of flavonoid bioactivities[J]. Chinese Journal of Applied & Environmental Biology,2006,12(2):283-286.
23. Tarahovsky Y S,Kim Y A,Yagolnik E A,et al. Flavonoid-membrane interactions:involvement of flavonoid-metal complexes in raft signaling[J]. Biochimica et Biophysica Acta(BBA)-Biomembranes,2014,1838(5):1235-1246.
24. 邓娇. 莲花瓣类黄酮色素分析及莲花瓣着色机理研究[D]. 武汉:中国科学院研究生院(武汉植物园),2015.Deng J. The analysis of flavonoid pigments in lotus petals and the studies on the coloration mechanism of lotus petals[D]. Wuhan:Wuhan botanical garden of the Chinese Academy of Sciences,2015.
25. Winkel-Shirley B. Flavonoid biosynthesis. a colorful model for genetics,biochemistry,cell biology,and biotechnology[J]. Plant Physiology,2001,126(2):485-493.
26. 许志茹,马静,崔国新,等. 芜菁类黄酮3'-羟化酶基因的功能鉴定及启动子初步分析[J]. 植物研究,2015,35(4):572-582.Xu Z R,Ma J,Cui G X,et al. Functional identification and promoter preliminary analysis of flavonoid 3'-hydroxylase genes in turnip[J]. Bulletin of Botanical Research,2015,35(4):572-582.
27. Boase M R,Lewis D H,Davies K M,et al. Isolation and antisense suppression of flavonoid 3'-5'-hydroxylase modifies flower pigments and colour in cyclamen[J]. BMC Plant Biology,2010,10:107.
28. Han Y P,Vimolmangkang S,Soria-Guerra R E,et al. Ectopic expression of apple F3'H genes contributes to anthocyanin accumulation in the Arabidopsis tt7 mutant grown under nitrogen stress[J]. Plant Physiology,2010,153(2):806-820.
29. Holton T A,Cornish E C. Genetics and biochemistry of anthocyanin biosynthesis[J]. The Plant Cell,1995,7(7):1071-1083.
30. Li X,Weng J K,Chapple C. Improvement of biomass through lignin modification[J]. The Plant Journal,2008,54(4):569-581.
31. Hoffmann L,Maury S,Martz F,et al. Purification,cloning,and properties of an acyltransferase controlling shikimate and quinate ester intermediates in phenylpropanoid metabolism[J]. The Journal of Biological Chemistry,2003,278(1):95-103.
32. Lepelley M,Cheminade G,Tremillon N,et al. Chlorogenic acid synthesis in coffee:an analysis of CGA content and real-time RT-PCR expression of HCT,HQT,C3H1,and CCoAOMT1 genes during grain development in C. canephora[J]. Plant Science,2007,172(5):978-996.
33. 曹运琳,邢梦云,徐昌杰,等. 植物黄酮醇生物合成及其调控研究进展[J]. 园艺学报,2018,45(1):177-192.Cao Y L,Xing M Y,Xu C J,et al. Biosynthesis of flavonol and its regulation in plants[J]. Acta Horticulturae Sinica,2018,45(1):177-192.
34. Winkel-Shirley B. Flavonoid biosynthesis. A colorful model for genetics,biochemistry,cell biology,and biotechnology[J]. Plant Physiology,2001,126(2):485-493.
35. Berim A,Gang D R. The roles of a flavone-6-hydroxylase and 7-O-demethylation in the flavone biosynthetic network of sweet basil[J]. The Journal of Biological Chemistry,2013,288(3):1795-1805.
36. Anzellotti D,Ibrahim R K. Molecular characterization and functional expression of flavonol 6-hydroxylase[J]. BMC Plant Biology,2004,4:20.
37. Latunde-Dada A O,Cabello-Hurtado F,Czittrich N,et al. Flavonoid 6-hydroxylase from soybean(Glycine max L. ),a novel plant P-450 monooxygenase[J]. Journal of Biological Chemistry,2001,276(3):1688-1695.
38. Berim A,Hyatt D C,Gang D R. A set of regioselective O-methyltransferases gives rise to the complex pattern of methoxylated flavones in sweet basil[J]. Plant Physiology,2012,160(2):1052-1069.
39. Overkamp S,Hein F,Barz W. Cloning and characterization of eight cytochrome P450 cDNAs from chickpea(Cicer arietinum L. ) cell suspension cultures[J]. Plant Science,2000,155(1):101-108.
40. Kochs G,Grisebach H. Enzymic synthesis of isoflavones[J]. The FEBS Journal,1986,155(2):311-318.
41. Hashim M F,Hakamatsuka T,Ebizuka Y,et al. Reaction mechamism of oxidative rearrangement of flavanone in isoflavone biosynthesis[J]. FEBS Letters,1990,271(1-2):219-222.
42. Hakamatsuka T,Mori K,Ishida S,et al. Purification of 2-hydroxyisoflavanone dehydratase from the cell cultures of pueraria lobata in honour of professor G. H. Neil towers 75TH birthday[J]. Phytochemistry,1998,49(2):497-505.
43. Koester J,Bussmann R,Barz W. Malonyl-coenzyme A:isoflavone 7-O-glucoside-6″-O-malonyltransferase from roots of chick pea(Cicer arietinum L. )[J]. Archives of Biochemistry and Biophysics,1984,234(2):513-521.
44. Akashi T,Aoki T,Ayabe S I. CYP81E1,a cytochrome P450 cDNA of licorice(Glycyrrhiza echinata L. ),encodes isoflavone 2'-hydroxylase[J]. Biochemical and Biophysical Research Communications,1998,251(1):67-70.
45. Chen G F,Huo Y S,Tan D X,et al. Melatonin in Chinese medicinal herbs[J]. Life Sciences,2003,73(1):19-26.
46. Tan D X,Hardeland R,Manchester L C,et al. Functional roles of melatonin in plants,and perspectives in nutritional and agricultural science[J]. Journal of Experimental Botany,2012,63(2):577-597.
47. 张娜. 褪黑素处理对渗透胁迫下黄瓜种子萌发及幼苗生长的影响及其分子机制[D]. 北京:中国农业大学,2014.Zhang N. Regulation of melatonin on germination and seedling growth under osmotic stress in cucumber[D]. Beijing:China Agricultural University,2014.
48. 耿庆伟,邢浩,郝桂梅,等. 外源褪黑素对臭氧胁迫下‘赤霞珠’葡萄叶片光合作用的影响[J]. 园艺学报,2016,43(8):1463-1472.Geng Q W,Xing H,Hao G M,et al. Effect of exogenous melatonin on photosynthesis of ‘Cabernet Sauvigon’ Grape leaves under ozone stress[J]. Acta Horticulturae Sinica,2016,43(8):1463-1472.

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