Effect of Exogenous H2O2 on Proline Accumulation and Metabolic Pathway in Leaves of Oat Seedlings under Complex Saline-Alkali Stress

doi:10.7525/j.issn.1673-5102.2016.03.017

Abstract

Abstract: Saline-alkali stress interferes with cell metabolism, and inhibits plant growth and development. Proline metabolism is closely related with plant salt-alkali resistance. As signaling molecules, hydrogen peroxide(H₂O₂) plays vital roles in the regulation of plant cell metabolism, as well as in adaptation to the environmental stress. In order to understand the regulatory mechanism of exogenous H₂O₂ on proline metabolism in oat(Avena nuda) under salinity-alkalinity stresses, the ‘Dingyou No.6'(a new oat cultivar) seedlings with two leaves were used to investigate the effects of exogenous H₂O₂ on proline accumulation in leaves of the seedlings under complex saline-alkali stress by the method of solution culture. The results showed that 75 mmol·L^-1 complex saline-alkali stress(molar ratio of NaCl:Na₂SO₄:NaHCO₃:Na₂CO₃=12:8:9:1) led to a significant accumulation of proline in leaves, and induced a rapid increase of activities of the key enzymes ornithine-δ-aminotransferase(δ-OAT) of proline biosynthesis, and a decrease of activities of the key enzymes Δ¹-pyrroline-5-carboxylate synthetase(P5CS) of proline biosynthesis, as well as the key enzyme proline dehydrogenase(ProDH) of proline degradation. Moreover, treatments with 0.01-1 000 μmol·L^-1, especially, 10 μmol·L^-1 H₂O₂ could enhance the accumulation of proline in oat seedling leaves under complex saline-alkali stress. H₂O₂ of 10 μmol·L^-1 also increased the activities of P5CS and δ-OAT, and decreased the activity of ProDH in leaves under complex saline-alkali stress. In addition, 10 μmol·L^-1 H₂O₂ treatments could rapidly increase the endogenous H₂O₂ levels in oat seedling leaves under complex saline-alkali stress. The exogenous H₂O₂ treatment resulted in the increase of endogenous H₂O₂ content in oat seedlings under complex saline-alkali stress. H₂O₂ induced proline accumulation might be a combined result of the activation of glutamate and ornithine pathways of proline biosynthesis and inhibition of proline degradation pathway.

Key words: oat, complex saline-alkali stress, hydrogen peroxide, proline metabolism

CLC Number:

Q945.78

LIU Jian-Xin, WANG Jin-Cheng, LIU Xiu-Li, WANG Feng-Qin. Effect of Exogenous H₂O₂ on Proline Accumulation and Metabolic Pathway in Leaves of Oat Seedlings under Complex Saline-Alkali Stress[J]. Bulletin of Botanical Research, 2016, 36(3): 427-433.

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Effect of Exogenous H₂O₂ on Proline Accumulation and Metabolic Pathway in Leaves of Oat Seedlings under Complex Saline-Alkali Stress

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