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Bulletin of Botanical Research ›› 2014, Vol. 34 ›› Issue (4): 561-566.doi: 10.7525/j.issn.1673-5102.2014.04.022

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Microwave-assisted Extraction of Paeoniflorin and Paeonol from Tree Peony Seed Coat and Content Determination

ZHAO Lian-Fei;CHEN Feng-Li;SUN Xi-Xiao;DU Xin-Qi;ZHAO Chun-Jian*;YAO Huan-Huan;YANG Lei;WANG Gui-Bin;ZU Yuan-Gang   

  1. 1.Key Laboratory of Forest Plant Ecology,Ministry of Education,Northeast Forestry University,Harbin　150040；2.College of Forest Resources and Environment,Nanjing Forestry University,Nanjing　210037
  • Received:1900-01-01 Revised:1900-01-01 Online:2014-07-20 Published:2014-07-20
  • Contact: ZHAO Chun-Jian
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Abstract: In this study, single factor experiment was used to optimize the main factors of microwave-assisted extraction for paeoniflorin and paeonol from tree peony seed coats. The optimum extraction conditions were as follows: ethanol extract fraction was 70%, liquid-solid ratio was 10∶1, microwave power was 230 W, microwave time was 10 min, soaking time was 1 h and extraction times was one. Under these conditions, the yields of paeoniflorin and paeonol were 2.88 and 0.52 mg·g-1, respectively. Meanwhile, the proposed method was validated in stability, repeatability and recovery experiments and the proposed method was a stable and efficiency method.

Key words: peony seed coats, Paeoniflorin, Paeonol, microwave-assisted extraction

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