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Bulletin of Botanical Research ›› 2018, Vol. 38 ›› Issue (5): 748-756.doi: 10.7525/j.issn.1673-5102.2018.05.015

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Principal Component and Subordinate Function of the Alleviating Effects of Hydrogen Peroxide(H2O2) on Low-temperature Stress in Naked Oat(Avena nuda) Seedlings

LIU Jian-Xin1,2, OU Xiao-Bin1,2, LIU Xiu-Li1,2, WANG Jin-Cheng1,2   

  1. 1. University Provincial Key Laboratory for Protection and Utilization of Longdong Bio-resources in Gansu Province, Gansu Province, Qingyang 745000;
    2. College of Life Sciences and Technology, Longdong University, Qingyang 745000
  • Received:2018-04-30 Online:2018-09-15 Published:2018-08-31
  • Supported by:
    The National Natural Science Foundation of China(31560125);The Science and Technology Plan Project of Qingyang City in Gansu Province(KZ2014-19)

Abstract: In order to explore whether H2O2 could improve the chilling tolerance in naked oat seedlings, three-leaf period ‘Dingyou No.6’ seedlings of sand culture were sprayed with 10 μmol·L-1 H2O2 for 12 h before 8℃/5℃(day/night) low temperature stress, spraying distilled water as the control, and 13 physiological indexes related to cold resistance including superoxide anion(O2), H2O2, malondialdehyde(MDA), superoxide dismutase(SOD), catalase(CAT), peroxidase(POD), ascorbic acid peroxidase(APX), ascorbic acid(AsA), glutathione(GSH), soluble sugar(SS), proline(Pro), soluble protein(SP) and heat stable protein(HSP) in the seedling leaves under low temperature stress for 0, 1, 2, 3, 4 and 5 d as well as the plant height and biomass increments of the seedlings processed for 5 d were determined, respectively. By principal component and subordinate function analyses, the effect of H2O2 on the cold resistance was evaluated in naked oat seedlings. Compared with the control, H2O2 treatment could significantly enhance the plant height increment and biomass increment of naked oat seedlings under low temperature stress, decrease O2 and MDA contents in leaves during low temperature stress and H2O2 content of low temperature stress for 2-5 d, and increase the activities of SOD, CAT, POD, APX and contents of AsA, GSH, SS, Pro, SP and HSP of naked oat seedling leaves. From 13 physiological indexes converted data matrix and principal component analysis, the cumulative variance contribution of four principal factors extracted was 85.6%. By subordinate function analysis, spraying H2O2 significantly improved the comprehensive evaluation value of the naked oat seedling leaves under low temperature stress for 0-5 d. Therefore, spraying H2O2 can improve the cold resistance of naked oat seedlings by regulating the physiological and biochemical metabolism.

Key words: H2O2, naked oat, chilling tolerance, principal components analysis, subordinate function

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