Abstract: We induced the callus from the leaves of Nicotiana benthamiana cultured in the MS medium and grew in the green house.Then we used the callus to detect the expression of BECTLIN Ⅰ gene which was correlated with Programmed Cell Death(PCD).The detection of RT-PCR showed that the fragment of BECTLIN Ⅰ gene cDNA was amplified in the leaves of the in-vitro plantlets subcultured in the medium and those of the plotted plant seedling, but BECTLIN Ⅰ gene expressed continually and steadily in the leaves callus of the plantlets.This probably indicated that auxins in the medium had some effect on the expression of BECTLIN Ⅰ gene.At the same time, PCD might occur in the course of the formation of the leaves callus, and in the state of dedifferentiation there probably appeared the embryoblasts with the same configuration and function.

Key words: Nicotiana benthamiana, callus, BECTLIN Ⅰ gene, RT-PCR, programmed cell death