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Bulletin of Botanical Research ›› 2020, Vol. 40 ›› Issue (1): 50-57.doi: 10.7525/j.issn.1673-5102.2020.01.008

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Establishing Tissue Culture and Regeneration System of Scutellaria regeliana

ZHANG Jing, NIU Zhe, FAN Wei-Fang, GAO Peng-Fei, WU Jian-Hui   

  1. College of Landscape Architecture, Northeast Forestry University, Harbin 150040
  • Received:2019-05-09 Online:2020-01-05 Published:2020-02-21
  • Supported by:
    Fundamental Research Business Expenses of Central Universities(2572019BK03)

Abstract: We studied the effects of different disinfectant treatments and different plant growth hormone ratios on axillary bud induction, callus induction, cluster bud differentiation, proliferation, rooting and transplantation of Scutellaria regeliana stem segments with the stem segments of S.regeliana as explants. The best disinfection method was 0.1% HgCl2 for 5 min, with the lowest pollution rate of 8.25%; the best medium for inducing axillary buds was MS+1 mg·L-1 6-BA+1 mg·L-1 NAA, with the induction rate of 73.66%; the best medium for inducing callus was MS+1 mg·L-1 6-BA+1 mg·L-1 2-4D, with the induction rate of 91.33%; and the best medium for inducing callus differentiation was MS+1 mg·L-1 6-BA+0.5 mg·L-1 NAA, with the differentiation rate of 44.71%. The optimum medium for bud multiplication was MS+1 mg·L-1 6-BA+0.5 mg·L-1 NAA, and the multiple of bud multiplication was 5.85; the optimum medium for rooting was 1/2MS+0.2 mg·L-1 IBA, and the rooting rate could reach 74.07%; the volume ratio of vermiculite:perlite:pearlite:garden soil was 1:1:3, the highest survival rate of transplantation was 79.24%, and the plant grew vigorously. In this study, the regeneration system of S.regeliana was established to provide theoretical support in the development and application of S.regeliana wild resources on the basis of proper protection.

Key words: Scutellaria regeliana, adventitious bud, callus, regeneration system

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