Cloning and sequencing analysis on the cDNA fragment SI800 separated from rye which related with salt tolerance

doi:10.7525/j.issn.1673-5102.2005.04.011

Bulletin of Botanical Research ›› 2005, Vol. 25 ›› Issue (4): 427-431.doi: 10.7525/j.issn.1673-5102.2005.04.011

Previous Articles Next Articles

Cloning and sequencing analysis on the cDNA fragment SI₈₀₀ separated from rye which related with salt tolerance

WANG Zhen-Ying¹, PENG Yong-Kang¹, ZHENG Jian-Yu²

1. Department of Biology, Tianjin Normal University, Tianjin 300074;
2. Department of Life Science, Nan Kai University, Tianjin 300071

Received:2004-12-20 Online:2005-12-15 Published:2016-06-14

Abstract

Abstract: SI₈₀₀ (Salt Induced(800 bp), SI₈₀₀) was a cDNA fragment, which separated from rye leaves by DDRT-PCR.In this study, we purified SI₈₀₀ by low temperature agarose electrophoresis and linked to pGEM-T vector then transformed to Escherichia coli(JM 109).30 positive clones were obtained.2 positive clones were selected randomly after test by digestion, PCR and sequenced.The result indicated that SI₈₀₀ was composed of(735 bp).DNA sequence analysis showed(68%) homologue to uxaB gene for altronate oxidoreductase in GenBank.

Key words: rye, salt tolerance, cloning of cDNA fragment, sequencing

WANG Zhen-Ying, PENG Yong-Kang, ZHENG Jian-Yu. Cloning and sequencing analysis on the cDNA fragment SI₈₀₀ separated from rye which related with salt tolerance[J]. Bulletin of Botanical Research, 2005, 25(4): 427-431.

[1]	Yijiao LIU, Jiqing ZHAO, Jinhuan CHEN. Cloning and Functional Study of the LrMYB64 Gene in Lyciumruthenicum [J]. Bulletin of Botanical Research, 2026, 46(2): 231-245.
[2]	Jingyifan ZHANG, Yi LI, Xinyi BI, Ji DE, Jifeng ZHANG, Xiaofang GUO. Diversity and Environmental Responses of the Rhizosphere Bacterial Community in Phragmites australis from the Lhalu Wetland, Xizang [J]. Bulletin of Botanical Research, 2026, 46(1): 181-194.
[3]	Lan WANG, Di ZHOU, Zhao XUE, Dongxu WANG, Xiaofang GUO, Ji DE. Dynamics of Endophytic Bacterial Communities in Highland Barley Seeds at Different Growth Stages [J]. Bulletin of Botanical Research, 2025, 45(6): 965-974.
[4]	Xinyu NI, Junying HE, Mengjiao YAN, Chao DU. Application Progress of RNA-Seq Technology in Rare and Endangered Plants [J]. Bulletin of Botanical Research, 2023, 43(4): 481-492.
[5]	Ziyin WANG, Bingru LIU, Zihao LI, Jifei WANG, Pengbin YANG. Genomic Survey Analysis of Endangered Plant Tetraena mongolica [J]. Bulletin of Botanical Research, 2023, 43(2): 186-193.
[6]	He-Xian JIN, Xu-Yue SHEN, Rong-Rong CHEN, Zhen WU, Ya-Mei SHEN, Dong-Mei ZHANG. Effects of NaCl Stress on Morphological and Physiological Characteristics of Magnolia denudata Desr. [J]. Bulletin of Botanical Research, 2021, 41(4): 596-603.
[7]	Yun TENG, Zong-Bo QIU, Wen-Li WANG, Xue-Ru ZHANG. Transcriptome Analysis in Vascular Cambium and Xylem of Paulownia tomentosa [J]. Bulletin of Botanical Research, 2021, 41(3): 354-361.
[8]	Chen-Chen ZHOU, Yi-Liang LI, Zhe WANG, Fen-Cheng ZHAO, Sui-Ying ZHONG, Chang-Ming LIN, Zhi-Qiang TAN, Fu-Ming LI, Hui-Shan WU, Wen-Bing GUO, Fang-Yan LIAO, Jian-Ge WANG. Screening and Expression Analysis of Different Genes for Oleoresin Production in the Specific Period of Pinus elliottii×P.caribaea by RNA-Seq Technology [J]. Bulletin of Botanical Research, 2021, 41(3): 419-428.
[9]	YANG Bin, MENG Qing-Yao, ZHANG Kai, DUAN Yi-Zhong. Chloroplast Genome Characterization and Identification of Genetic Relationship of Relict Endangered Plant Amygdalus nana [J]. Bulletin of Botanical Research, 2020, 40(5): 686-695.
[10]	ZHANG Yu, XIA Ming-Ze, ZHANG Fa-Qi. Transcriptome Analysis for Medicinal Plant Anisodus tanguticus [J]. Bulletin of Botanical Research, 2020, 40(3): 458-467.
[11]	LI Zi-Yi, HE Zi-Hang, LU Hui-Jun, WANG Yu-Cheng, JI Xiao-Yu. Study on Salt Tolerance of AtUNE12 Gene in Arabidopsis thaliana [J]. Bulletin of Botanical Research, 2020, 40(2): 257-265.
[12]	XIONG Yang-Yang, LUO Lin-Li, ZHAO Shi-Hao, ZHOU An-Long, WANG Jin. Transcriptome of Single and Double Tepals of Clematis patens [J]. Bulletin of Botanical Research, 2020, 40(1): 93-105.
[13]	ZHANG Shan-Shan, KANG Hong-Mei, YANG Wen-Zhong. Population Genetic Analysis of Nyssa yunnanensis by Reduced-representation Sequencing Technique [J]. Bulletin of Botanical Research, 2019, 39(6): 899-907.
[14]	YANG Yun, CHEN Meng-Jiao, DU Qing-Xin, ZHU Jing-Le, DU Hong-Yan, YANG Shao-Bin. SNP Sites Developed by Whole Genome Resequencing Analysis in Eucommia ulmoides ‘Hongye’ [J]. Bulletin of Botanical Research, 2019, 39(6): 947-954.
[15]	DUAN Yi-Zhong, DU Zhong-Yu, WANG Hai-Tao. Chloroplast Genome Characteristics of Endangered Relict Plant Tetraena mongolica in the Arid Region of Northwest China [J]. Bulletin of Botanical Research, 2019, 39(5): 653-663.

Cloning and sequencing analysis on the cDNA fragment SI₈₀₀ separated from rye which related with salt tolerance

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments