ANALYSIS OF RESTRICTION ENZYMES OF CHLOROPLAST DNA IN TOMATO VARIETIES WHICH HAVE DIFFEERENT RESISTANCE TO TOBBACCO MOSSIC VIRUS

Bulletin of Botanical Research ›› 1995, Vol. 15 ›› Issue (3): 391-396.

Previous Articles Next Articles

ANALYSIS OF RESTRICTION ENZYMES OF CHLOROPLAST DNA IN TOMATO VARIETIES WHICH HAVE DIFFEERENT RESISTANCE TO TOBBACCO MOSSIC VIRUS

Huang Yong-fen¹, Wang Qing yin¹, Sun De jun¹, Fu Gui rong¹, Wang Hat ting¹, Xing Yi¹, Cheng Zhen-qi²

1. Department of Biology, Harbin Normal University, Harbin, 150080;
2. Department of Biological Science and Technology, Tsinghua University, Beijing 100084

Received:1995-02-01 Online:1995-09-15 Published:2016-06-14

Abstract

Abstract: We analyze ct-DNA of tomato which is resistant and sensitive to TMV with restriction enzyme. Our electrophoretogram of BamHI, EcoRI. Pst I is similar to other report.Molecular weight of ct-DNA is 156. 9kb, it is similar to others' study.We find some difference in BamHI EcoRI, PSt I electrophoretogram between resistant group and sensitive group. We guess this maybe the alternation of base sequence or insertion or deletion of short DNA sequence. We also infer that nucleus genome and chloroplast genome together decide the resistance of plant to Tobbacco Mossic Virus.

Key words: Tomato, TMV, ct-DNA, Restriction endonucleases

Huang Yong-fen, Wang Qing yin, Sun De jun, Fu Gui rong, Wang Hat ting, Xing Yi, Cheng Zhen-qi. ANALYSIS OF RESTRICTION ENZYMES OF CHLOROPLAST DNA IN TOMATO VARIETIES WHICH HAVE DIFFEERENT RESISTANCE TO TOBBACCO MOSSIC VIRUS[J]. Bulletin of Botanical Research, 1995, 15(3): 391-396.

[1]	Luhua ZHOU, Junyi FANG, Zimo XIONG, Weifeng WU, Jiarui LIU, Qiao LU, Hongqing LING, Danyu KONG. Evaluation on Waterlogging Tolerance of Different Tomato Germplasm [J]. Bulletin of Botanical Research, 2023, 43(5): 657-666.
[2]	SONG Fang-Yuan1；SU Hong-Yan2；CHENG Xian-Hao2；ZHU Lu-Ying2；WANG Lei1. Characterization and Expression Analysis of TNAC* Genes of Tomato [J]. Bulletin of Botanical Research, 2015, 35(6): 898-903.
[3]	LIU Xia;HU Zong-Li;ZHANG Yan-Jie;ZHU Ming-Ku;YIN Wen-Cheng;CHEN Guo-Ping. Cloning,Bioinformatics and Expression Analysis of SlMYBL* Gene in Tomato [J]. Bulletin of Botanical Research, 2014, 34(5): 664-670.
[4]	CAO Ying;TANG Xiao-Feng;LIU Yong-Sheng. Cloning, Expression Pattern and Bioinformation Analyses of COBRA Gene in Tomato(Solanum lycopersicum) [J]. Bulletin of Botanical Research, 2012, 32(3): 304-310.
[5]	HE Xiu-Xia;YU Yuan-Hua;GUO Hong-Yu;ZHANG Shu-Hua. A Effective Preparation Method for DNA from Gene Silencing Plant Induced by Virus [J]. Bulletin of Botanical Research, 2008, 28(6): 726-729.
[6]	LONG Jiao-Yue;HU Zong-Li;CHEN Xu-Qing;YAN Bo;CHEN Guo-Ping. Molecular Cloning and Characterization of LeEBF1 and LeEBF*2 from Tomato [J]. Bulletin of Botanical Research, 2008, 28(6): 710-715.
[7]	LÜFeng-Xia;GUO Zhao-Kui;YAN Pei-Qiang;WAN Xiu-Qing;PAN Yong-Ming. Construction of TMV-replicase Gene-targeted Vectoer for RNA Interference [J]. Bulletin of Botanical Research, 2008, 28(3): 310-314.
[8]	SHANG Sang;TIAN Li-Bo;HUANG Mian-Jia;CAI Jie. Effect of Simulated Acid Rain on Seed Germination of Three Solanaceous Vegetables [J]. , 2007, 27(4): 494-499.
[9]	LIU Hui-Min;ZHU Yue-Lin*;CHEN Lei. In Virto Comparision of Ca(NO₃)₂ Tolerance of Different Tomato Cultivars and Rootstock Selfed Lines at Seedling Stage [J]. Bulletin of Botanical Research, 2007, 27(2): 175-181.
[10]	GUO Zhi-ping. RESEARCH OF TUBE STORAGE TECHNIQUE AND ITS EFFECTS OF TABACCO CONTAINED VIRUSES [J]. Bulletin of Botanical Research, 2002, 22(3): 333-336.
[11]	Mi Xiao-ju, Ma Yue, Guo Gui-yun. STUDY ON THE EFFECT OF TOMATO SEEDS PHYSIOLOGY AND BIOCHEMISTRY WITH MAGNETIC FIELD TREATMENT [J]. Bulletin of Botanical Research, 1999, 19(1): 68-74.
[12]	Feng Yu-long, Liu En-ju, Cui Zhen-xiang. INFLUENCE OF TEMPERATURE OF ROOT SYSTEM ON TOMATO(Ⅱ)——INFLUENCE OF ROOT TEMPERATURE ON PHOTOSYNTHESIS AND WATER METABOLISM OF TOMATO [J]. Bulletin of Botanical Research, 1996, 16(2): 214-218.
[13]	Cai Su-wen, Guo Gui-yun, Zhang Hong-mei, Xi Jiang-hua. CHANGES IN ACTIVITY OF PROTECTIVE ENZYME OF TOMATO SEEDLING WITH THE SEEDS TREATED BY MAGNETIC FIELD UNDER CHILLING STRESS [J]. Bulletin of Botanical Research, 1996, 16(2): 219-223.
[14]	Guo Gui-yun, Wang Yan-ying, Liu Zhen-dong, Mi Xiao-ju, Wang Gui-ling, Xie Gui-qin. EFFECTS OF ⁶⁰Co-r RAY RADIATION ON TOMATO'S ROOT TIP CELLS WITH RADIATING TOMATO SEEDS [J]. Bulletin of Botanical Research, 1996, 16(1): 108-113.
[15]	Wang Qing-yin, Fu Gui-rong, Liu Yan-xia, Huang Yong-fen, Zhang Hong-gang, Xie Wei-wu, Wang Bin. THE ANALYSIS OF ct-DNA RESISTANT AND SENSITIVE TO TMV WITH RAPD IN TOMATO VARIETIES [J]. Bulletin of Botanical Research, 1996, 16(1): 114-117.

ANALYSIS OF RESTRICTION ENZYMES OF CHLOROPLAST DNA IN TOMATO VARIETIES WHICH HAVE DIFFEERENT RESISTANCE TO TOBBACCO MOSSIC VIRUS

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments