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Bulletin of Botanical Research ›› 2014, Vol. 34 ›› Issue (5): 664-670.doi: 10.7525/j.issn.1673-5102.2014.05.013

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Cloning,Bioinformatics and Expression Analysis of SlMYBL Gene in Tomato

LIU Xia;HU Zong-Li;ZHANG Yan-Jie;ZHU Ming-Ku;YIN Wen-Cheng;CHEN Guo-Ping*   

  1. College of Bioengineering,Chongqing University,Chongqing 400044
  • Received:1900-01-01 Revised:1900-01-01 Online:2014-09-20 Published:2015-03-19
  • Contact: CHEN Guo-Ping
  • Supported by:

Abstract: MYB proteins comprise one of the largest families of transcription factors in plants and are involved in plant growth and metabolism. According to the tomato sequences in the NCBI database, a 1 630 bp DNA fragment was cloned by RT-PCR from wild-type tomato AC++, and its opening reading-frame (ORF) was 1 245 bp, encoding 414 amino acids with a conservative DNA binding domain: MYB-like, C-terminal end and multiple phosphorylation sites, named SlMYBL. Bioinformatics analysis indicated that SlMYBL protein might be localized in the mitochondrial matrix and cytoplasm. Secondary structure prediction suggested that SlMYBL protein contains 26.33% alpha helix, 65.46% of no rules curl and 6.28% of the extended chain and 1.93% beta collapse. Real-time fluorescent quantitative PCR analysis showed that SlMYBL was highly expressed in mature leaves, stems, and sepals comparing with other tissues, and was induced by exogenous ethylene precursor IAA, ABA. In addition, the expression of SlMYBL was also induced under high and low temperatures, injury and salt stress. These results lay a foundation for further study on function of the SlMYBL gene in the process of growth and development of tomato.

Key words: SlMYBL, tomatoe, bio-informatics, hormone treatment, abiotic stress

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