Genetic Structure and Phylogeography Analysis of Artemisia intramongolica Based on Single Copy Nuclear Genes

doi:10.7525/j.issn.1673-5102.2022.06.005

Abstract

Abstract:

To investigate the genetic and phylogeography structure of Artemisia intramongolica， which was an important sand-fixing plant in Hunsandak sandy land in China， the homologous single-copy nuclear genes c9065 and c7847 were developed by transcriptome sequencing， and then eight natural populations of A. intramongolica were studied. The results showed that the total length of c9065 and c7847 were 485 bp and 457 bp， respectively， with 14 and 19 variable loci， and 48 and 40 haplotypes were obtained. Based on c9065 and c7847， the haplotype diversity（H_d） was 0.871 6 and 0.934 8， respectively， and all populations were above 0.8. The total nucleotide diversity（π） was 0.008 2 and 0.005 9， respectively， and all populations were above 0.005， indicating that both species and populations had high genetic diversity. The results of AMOVA analysis showed that 99.398% and 98.908% of genetic variation existed within the populations of A. intramongolica based on c9065 and c7847， respectively， with gene flow（N_m）of 6.810 and 7.270， respectively， much greater than 1， indicating that there was extensive gene exchange among populations of A. intramongolica. Based on c9065， N_st<G_st， although N_st>G_st by c7847， but the difference was not significant（P>0.05）， indicating that A.intramongolica population did not exist phylogeographic structure. The neutral test showed that Tajima’s D values for both nuclear geneswere all not significant（P>0.05）， while Fu’s F_s values were all significant（P<0.05）， and combined with the mismatch distribution analysis， A.intramongolica experienced limited population expansion under the neutral mode.

Key words: Artemisia intramongolica, single-copy nuclear genes, genetic structure, phylogeography

CLC Number:

Q948.2

Siru TANG, Tiejuan WANG, Fang XIU, Mengyao LIU, Hui ZHANG. Genetic Structure and Phylogeography Analysis of Artemisia intramongolica Based on Single Copy Nuclear Genes[J]. Bulletin of Botanical Research, 2022, 42(6): 956-963.

Figures/Tables 8

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种群代号 Code	采样地点 Sample location	凭证标本号 Voucher specimen	经度 Longitude（E）	纬度 Latitude（N）	生境 Habitat
XSQ	内蒙古苏尼特右旗 Sunit Right Banner，Inner Mongolia	S7703	112˚53′28″	43˚6′12″	固定沙地 Fixed sand
BLK	内蒙古锡林浩特市贝力克牧场 Belik Ranch，Xilinhot City，Inner Mongolia	B7613	116˚8′49″	43˚35′58″	固定沙地 Fixed sand
WRT	内蒙古正蓝旗乌日图 Wuritu，Zhenglan Banner，Inner Mongolia	W7708	115˚59′19″	43˚1′46″	固定沙地 Fixed sand
SGDL	内蒙古正蓝旗桑根达来 Sangendalai， Zhenglan Banner，Inner Mongolia	S6301	115˚57′12″	42˚40′20″	固定沙地 Fixed sand
BQ	内蒙古正镶白旗 Zhengxiangbai Banner，Inner Mongolia	B7725	114˚48′37″	42˚37′	半固定沙地 Semi-fixed sand
BYXL	内蒙古锡林浩特市白音锡勒牧场 Baiyinxile Ranch，Xilinhot City，Inner Mongolia	X7711	116˚42′16″	43˚37′50″	固定沙地 Fixed sand
SY	内蒙古克什克腾旗三义 Sanyi，Keshketeng Banner，Inner Mongolia	S7608	117˚15′49″	43˚12′47″	固定沙地 Fixed sand
XWQ	内蒙古西乌珠穆沁旗 West Ujimqin Banner，Inner Mongolia	X1932	117˚35′14″	44˚36′21″	固定沙地 Fixed sand

核基因类型 Nuclear gene types	变异来源 Source of variation	自由度 df	平方和 Sum of squares	变异成分 Variance components	变异比率 Percentage of variation /%
c9065	种群间 Among populations	7	16.265	0.012	0.602
	种群内 Within populations	224	442.753	1.977	99.398
	总计Total	231	459.017	1.989
c7847	种群间 Among populations	7	13.119	0.015	1.093
	种群内 Within populations	286	381.436	1.334	98.908
	总计Total	293	394.554	1.348

种群 Pop.	c9065				c7847
种群 Pop.	Tajima’s D	Fu’s F_s	SSD	指数 Raggedness index	Tajima’s D	Fu’s F_s	SSD	粗糙度指数 Raggedness index
整体Total	1.753	-25.540*	0.006	0.013	-0.288	-25.483*	0.002*	0.035*
XSQ	0.930	-2.780	0.033	0.054	-0.713	-6.794*	0.003	0.032
BLK	1.921	-2.029	0.024	0.058	0.179	-10.170*	0.019*	0.081*
WRT	1.035	-4.416*	0.013	0.021	-0.173	-12.412*	0.002	0.045*
SGDL	1.687	-4.335*	0.015	0.057	0.107	-9.048*	0.009*	0.043*
BQ	1.563	-1.342	0.028	0.049	0.567	-8.659*	0.009*	0.054*
BYXL	0.848	-5.960*	0.011	0.026	0.932	-5.581*	0.005	0.023
SY	1.220	-8.918*	0.006	0.014	0.482	-12.219*	0.021*	0.082*
XWQ	1.237	-4.407*	0.088	0.028	0.199	-8.836*	0.003	0.019

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