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Bulletin of Botanical Research ›› 2019, Vol. 39 ›› Issue (4): 539-546.doi: 10.7525/j.issn.1673-5102.2019.04.008

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Cloning and Expression Analysis of Catalase Gene(LoCAT1) from Larix olgensis

BAI Xiao-Ming, DONG Shi-Wei, YANG Yu-Ning, SONG Yue, ZHANG Han-Guo, LI Shu-Juan   

  1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040
  • Received:2019-01-22 Online:2019-07-05 Published:2019-07-03
  • Supported by:
    Heilongjiang science fund project(C2017008);New strains of fast-growing drought-resistant transgenic larch were cultivated in northeast China(2018ZX08020-003-001)

Abstract: In order to know about genes of Larix olgensis catalase and determine the expression characteristics in different organs of L. olgensis under different stresses, a CAT1 gene was cloned based on specific primers designed according to the CAT1 gene sequences from transcriptome database of L. olgensis, named LoCAT1. The complete length of open read frame(ORF) is 954 bp, encoding 317 amino acids. The results of phylogenetic tree analysis indicated that there was a closer relationship between LoCAT1 and Picea sitchensisand Ginkgo biloba. By real-time quantitative polymerase chain reaction (qPCR) analysis, LoCAT1 is highly expressed in leaf, while less expressed in stems. The expressions of LoCAT1 were obviously difference in roots and stems and leaves of L. olgensis under abiotic stress. The expression patterns of LoCAT1 were not exactly similarity among these treatments. The expression levels of LoCAT1 in roots and stems of L. olgensis after the treatment of NaCl were down-regulated, with the lowest expression at 12 h. While expression level in leaves was significantly inhibited at 24 h, and then up-regulated, with the highest expression at 96 h. The expression levels of LoCAT1 in roots and stems of L. olgensis after the treatment of PEG6000 were significantly inhibited in the early stage and then up-regulated. While LoCAT1 expression level in leaves was up-regulated. It was suggested that LoCAT1 may play an important role in response to stresses in plant.

Key words: Larix olgensis, LoCAT1, cloning, bioinformatics analysis, expression analysis

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